A rapid and sensitive method for hydroxyl radical detection on a microfluidic chip using an N-doped porous carbon nanofiber modified pencil graphite electrode
A rapid and sensitive method for hydroxyl radical detection on a microfluidic chip using an N-doped porous carbon nanofiber modified pencil graphite electrode
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使用氮掺杂多孔碳纳米纤维修饰铅笔石墨电极在微流控芯片上快速灵敏地检测羟基自由基的方法
DOI：
10.1039/c4an00471j
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发表时间：
2014-01-01
期刊：
影响因子：
4.2
通讯作者：
Zhou, Guo-Jun
中科院分区：
文献类型：
--
作者：
Ouyang, Jun;Li, Zhong-Qiu;Zhou, Guo-Jun
羟基自由基（(OH)- o -中心点）在人类疾病中起着重要作用。传统的检测方法既耗时又需要昂贵的仪器。本文提出了一种利用电化学技术检测微流控芯片上羟基自由基的简便、灵敏方法。将苯胺单体在铅笔石墨电极表面进行电化学聚合，并在800℃下碳化，得到的n掺杂多孔碳纳米纤维修饰铅笔石墨电极直接嵌入微流控芯片中作为工作电极。选择4-羟基苯甲酸（4-HBA）作为捕集剂，是因为它具有独特的3,4- dhba产物，捕集效率高。在微流控芯片上实现了1.0 x 10(-6) M的低检测限。作为示范，该微流控芯片成功地用于香烟烟雾中(OH)- o中心点的检测。氮掺杂碳材料与铅笔石墨之间的强pi-pi堆叠和疏水相互作用使改性电极非常适合微流控芯片。
Hydroxyl radicals ((OH)-O-center dot) play an important role in human diseases. Traditional detection methods are time consuming and require expensive instruments. Here, we present a simple and sensitive method for the detection of hydroxyl radicals on amicrofluidic chip using an electrochemical technique. Aniline monomer is electrochemically polymerized on the surface of a pencil graphite electrode and carbonized at 800 degrees C. The resulting N-doped porous carbon nanofiber-modified pencil graphite electrode is embedded into a microfluidic chip directly as a working electrode. 4-Hydroxybenzoic acid (4-HBA) is selected as the trapping agent owing to its unique 3,4-DHBA product and high trapping efficiency. A low detection limit of 1.0 x 10(-6) M is achieved on the microfluidic chip. As a demonstration the microfluidic chip is successfully utilized for the detection of (OH)-O-center dot in cigarette smoke. The strong pi-pi stacking and hydrophobic interactions between the nitrogen-doped carbon materials and the pencil graphite make the modified electrode well-suited for the microfluidic chip.