Quantification of proteins in the low nanogram range by staining with the colloidal gold stain AuroDye.
Quantification of proteins in the low nanogram range by staining with the colloidal gold stain AuroDye.
复制标题
通过使用胶体金染色剂 AuroDye 染色，对低纳克范围内的蛋白质进行定量。
DOI：
10.1016/0003-2697(87)90515-x
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发表时间：
1987
影响因子：
2.9
通讯作者：
S. Hunter
中科院分区：
文献类型：
--
作者：
J. Hunter;S. Hunter
一个简单的测定蛋白质在低纳克范围内描述。将蛋白质以条带形式结合到硝酸纤维素纸上，用市售胶体金染料AuroDye（Jansen Life Sciences Products的注册商标）染色，并通过扫描光密度测定法定量：可以一致地测量1.25至20 ng蛋白质。不同蛋白质的染色强度的变化进行了讨论。DNA、RNA、聚乙烯吡咯烷酮、Ficoll和6 M尿素不影响测定，并且可以测定在含有0.1%十二烷基硫酸钠的简单裂解缓冲液中的细胞材料样品。
A simple assay for proteins in the low nanogram range is described. Proteins are bound to nitrocellulose paper in a band, stained with the commercially available colloidal gold stain AuroDye (a registered trademark of Jansen Life Sciences Products), and quantified by scanning densitometry: 1.25 to 20 ng of protein can be consistently measured. Variation in the staining intensity of different proteins is discussed. DNA, RNA, polyvinylpyrrolidone, Ficoll, and 6 m urea do not affect the assays, and samples from cellular material in a simple lysis buffer containing 0.1% of sodium dodecyl sulfate can be assayed.
影响因子：
2.9
作者：
Zawieja,D;Barber,BJ;Roman,RJ
通讯作者：
Roman,RJ