(18)F-labeled-bioorthogonal liposomes for in vivo targeting.
(18)F-labeled-bioorthogonal liposomes for in vivo targeting.
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DOI：
10.1021/bc400322h
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发表时间：
2013-11-20
影响因子：
4.7
通讯作者：
Reiner, Thomas
中科院分区：
文献类型：
--
作者：
Emmetiere, Fabien;Irwin, Christopher;Viola-Villegas, Nerissa Therese;Longo, Valerie;Cheal, Sarah M.;Zanzonico, Pat;Pillarsetty, NagaVaraKishore;Weber, Wolfgang A.;Lewis, Jason S.;Reiner, Thomas
Liposomes are attractive vehicles for the controlled release of drugs and cytotoxins and have a long-standing history in medical research and clinical practice. In addition to established therapeutic indications, liposomes have several favorable properties for molecular imaging, including high stability and the ability to be labeled with radioisotopes as well as paramagnetic and fluorescent contrast agents. However, long circulation times and difficulties in creating targeted liposomes have proven challenges for imaging. In this study, we have addressed these limitations using a recently developed strategy for bioorthogonal conjugation, the reaction between tetrazines and trans-cyclooctenes. By coating radiolabeled liposomes with trans-cyclooctene and pretargeting with a tetrazine coupled to a targeted peptide, we were able to selectively enhance the retention of liposomes and bind them to tumor tissue in live animals. The rapid reaction between tetrazines and trans-cyclooctenes allowed imaging to be performed with the short-lived PET tracer 18F, yielding signal-to-background activity ratios of 7:1. The covalent, bioorthogonally-driven tumor-targeting of liposomes by in vivo click chemistry is promising and should be explored for more selective and rapid delivery of radiodiagnostics and radiotherapeutics, two classes of drugs which particularly benefit from fast clearance, low non-specific binding, and the associated reduced toxicity to kidneys and bone marrow.
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影响因子：
15
作者：
Blackman, Melissa L.;Royzen, Maksim;Fox, Joseph M.
通讯作者：
Fox, Joseph M.
DOI：
10.1073/pnas.1113466109
发表时间：
2012-03-27
影响因子：
11.1
作者：
Devaraj, Neal K.;Thurber, Greg M.;Weissleder, Ralph
通讯作者：
Weissleder, Ralph
影响因子：
5.6
作者：
Segala J;Engelman DM;Reshetnyak YK;Andreev OA
通讯作者：
Andreev OA
DOI：
10.2967/jnumed.112.115840
发表时间：
2013-08
期刊：
Journal of nuclear medicine : official publication, Society of Nuclear Medicine
影响因子：
--
作者：
Zeglis BM;Sevak KK;Reiner T;Mohindra P;Carlin SD;Zanzonico P;Weissleder R;Lewis JS
通讯作者：
Lewis JS
影响因子：
16.6
作者：
Yang, Jun;Karver, Mark R.;Li, Weilong;Sahu, Swagat;Devaraj, Neal K.
通讯作者：
Devaraj, Neal K.