Whole-genome sequencing and comparative transcriptome analysis of Bombyx mori nucleopolyhedrovirus La strain
Whole-genome sequencing and comparative transcriptome analysis of Bombyx mori nucleopolyhedrovirus La strain
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DOI：
10.1007/s11262-019-01727-2
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发表时间：
2020-04-01
期刊：
影响因子：
1.6
通讯作者：
Iwanaga, Masashi
中科院分区：
文献类型：
--
作者：
Fujimoto, Shota;Kawamoto, Munetaka;Iwanaga, Masashi
家蚕核多角体病毒（BmNPV） La是在老挝分离的一种变型BmNPV毒株。La在毒力、多面蛋白的产生和多衣壳封闭衍生病毒的形成等方面与BmNPV型株T3有不同的特点。本文将La的全基因组序列与9株BmNPV和2株家蚕核多角体病毒的序列进行了比较。完整的La基因组包含127,618个碱基对，G + C含量为40.3%，包含136个开放阅读框，编码60多个氨基酸。La基因组缺少bro-b基因，与T3的同源性最高。La-和T3感染细胞的转录组比较表明，La感染细胞中多角蛋白和组织蛋白酶基因的表达水平高于T3感染细胞。有趣的是，不同RNA水平的病毒基因在La的基因组中被组装成5个簇。此外，La感染的细胞中宿主核糖体蛋白基因RNA水平明显低于T3感染的细胞。
The Bombyx mori nucleopolyhedrovirus (BmNPV) La is a variant BmNPV strain isolated in Laos. La has different features from BmNPV type strain T3 in virulence, production of the polyhedrin protein, and the formation of multicapsid occlusion-derived viruses. Here, the whole-genome sequence of La was compared to the sequences of nine BmNPV and two Bombyx mandarina nucleopolyhedrovirus strains. The complete La genome consisted of 127,618 base pairs with a G + C content of 40.3% and contained putative 136 open reading frames encoding more than 60 amino acids. The La genome lacked the bro-b gene and had the highest identity with that of the T3 strain. A comparison of the transcriptomes of La- and T3-infected cells showed that the expression levels of the polyhedrin and cathepsin genes were greater in cells infected with La as compared to those infected with T3. Interestingly, the virus genes with different RNA levels between the two BmNPV strains were assembled into five clusters in the genome of La. Also, the RNA levels of host ribosomal protein genes were significantly decreased in cells infected with La as compared to those infected with T3.