Study on the Resistance to Fluoroquinolones and GyrA Mutation of Salmonella from Animal
Study on the Resistance to Fluoroquinolones and GyrA Mutation of Salmonella from Animal
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发表时间：
2009
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通讯作者：
Xiong Zhong-liang
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作者：
Xiong Zhong-liang
采用微量稀释法和纸片扩散法检测动物源沙门氏菌对氟喹诺酮类药物的耐药性。PCR扩增GyrA基因的喹诺酮类药物耐药决定区（QRDR），获得487 bp的特异性片段。PCR产物直接测序，用DNAstar软件进行序列比对。结果表明，盐酸环丙沙星、恩诺沙星、氧氟沙星、甲磺酸培氟沙星、烟酸诺氟沙星的GyrA突变浓度分别为16~ 128、32 ~ 128、32 ~ 256、128 ~512和64~512 μg.mL-1。结果表明，受试沙门氏菌对氟喹诺酮类药物存在多重耐药，GyrA基因QRDR突变形式多样。
Microdilution and disc diffusion techniques were used to determine the resistance to fluoroquinolones of Salmonella from animal.A 487bp specific fragment was obtained after PCR which was performed to amplify Quinolone resistance determining regions(QRDR) of GyrA.PCR products were directly sequenced and the alignment of sequences was done with software DNAstar.The results showed that the MIC ranges of ciprofloxacin hydrochloride,enrofloxacin,ofloxacin,pefloxacin mesylate and norfloxacin nicotinate were 16～128,32～128,32～256,128～512 and 64～512 μg.mL-1 respectively.The mutation sites of GyrA were found as follows: Ser83→Phe(12/15),Asp87→Asn(10/15),Asp87→Tyr(5/15),Ser83→Gly(3/15) and Ala119→Val(1/15).All the results showed that Salmonella in the test were multi-resistance to fluoroquinolones and mutations in QRDR of GyrA showed many forms.