SEQUENCES THAT REGULATE THE DIVERGENT GAL1-GAL10 PROMOTER IN SACCHAROMYCES-CEREVISIAE
SEQUENCES THAT REGULATE THE DIVERGENT GAL1-GAL10 PROMOTER IN SACCHAROMYCES-CEREVISIAE
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DOI：
10.1128/mcb.4.8.1440
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发表时间：
1984-01-01
影响因子：
5.3
通讯作者：
DAVIS, RW
中科院分区：
文献类型：
--
作者：
JOHNSTON, M;DAVIS, RW
The GAL1 and GAL10 genes of S. cerevisiae are divergently transcribed, with 606 base pairs of DNA separating their transcription initiation sites. These 2 genes are stringently coregulated: their expression is induced .apprx. 1000-fold in cells growing on galactose and is repressed by growth on glucose. The nucleotide sequence of the region of DNA between these genes and the precise sites of transcription initiation are presented here. The most notable feature of the nucleotide sequence of this region is a 108-base-pair guanine-plus-cytosine-rich stretch of DNA located approximately in the middle of the region between GAL1 and GAL10. Analysis of the effects of mutations that alter the region between these 2 genes, constructed in vitro or selected in vivo, suggest that these guanine-plus-cytosine-rich sequences are required for the expression of both genes. The region of DNA between GAL1 and GAL10 is sufficient for regulation of expression of these genes: fusion of the region to the yeast HIS3 gene places HIS3 under GAL control.
