Temporal and spatial expression pattern of β1 sodium channel subunit during heart development
Temporal and spatial expression pattern of β1 sodium channel subunit during heart development
复制标题
DOI：
10.1016/j.cardiores.2004.11.028
复制
发表时间：
2005-03-01
影响因子：
10.8
通讯作者：
Aránega, AE
中科院分区：
文献类型：
--
作者：
Domínguez, JN;Navarro, F;Aránega, AE
目的：研究小鼠心脏发育过程中心孔形成Na+通道亚单位调控因子Scn1b基因的表达水平和蛋白分布。方法：采用实时定量RT-PCR方法检测胚胎心脏(E9.5~E18.5)以及出生后和成年心脏中Scn1b基因的表达水平。结果：Scn1b基因表达呈动态变化，在E12.5期达高峰，E15.5期达最低水平。Scn1b基因在胚胎和新生儿发育后期均有表达，在成人心脏中表达最强。免疫组织化学实验显示，在胚胎早期，Scn1b蛋白在不同心腔中的分布相似。随着发育的深入，Scn1b蛋白在小梁状心肌和束支中的表达增强。在胚胎后期和出生后小鼠心肌细胞的亚细胞水平上，α-肌动蛋白免疫染色发现Scn1b存在于T管，连接蛋白43免疫染色证实Scn1b存在于间盘。结论：Scn1b在小鼠心脏发育过程中有表达，提示Scn1b在心脏形态发生过程中对心肌细胞的动作电位结构可能起重要作用。(C)2004年欧洲心脏病学会。爱思唯尔出版，版权所有。
Objectives: The aim of this study is to analyze Scn1b mRNA expression levels and protein distribution of Scn1b, a putative modulator of the pore-forming Na+ channel subunit in the heart, during mouse cardiac development.Methods: Scn1b mRNA levels were determined by real-time RT-PCR using embryonic hearts ranging from E9.5 to E18.5 as well as in postnatal and adult heart. Scn1b protein distribution and subcellular localization during cardiogenesis were analyzed by immunohistochemistry and confocal microscopy.Results: Scn1b mRNA showed a dynamic expression pattern, peaking at stage E12.5 and decreasing at E15.5. Scn1b mRNA increased at later embryonic and neonatal stages, being maximal in the adult heart. Immunohistochemistry experiments revealed comparable distribution of Scn1b protein between the different cardiac chambers at early embryonic stages. With further development, Scn1b protein showed an enhanced expression in the trabeculated myocardium and the bundle branches. At the subcellular level in later embryonic and postnatal mouse cardiomyocytes, Scn1b was present in T-tubules as identified by immunostaining of alpha-actinin, and in the intercalated disks as identified by immunostaining of connexin 43.Conclusion: These results demonstrate that Scn1b is expressed during mouse heart development, suggesting it can play an important role in the action potential configuration of the cardiomyocytes during heart morphogenesis. (C) 2004 European Society of Cardiology. Published by Elsevier B.V. All rights reserved.