DYNAMICS OF NA,K-ATPASE SITES IN LATERAL COCHLEAR WALL TISSUES OF THE RAT
DYNAMICS OF NA,K-ATPASE SITES IN LATERAL COCHLEAR WALL TISSUES OF THE RAT
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DOI：
10.1007/bf00186223
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发表时间：
1993-09-01
影响因子：
2.6
通讯作者：
RAREY, KE
中科院分区：
文献类型：
--
作者：
CURTIS, LM;TENCATE, WJF;RAREY, KE
本研究的目的是确定是否不同水平的合成糖皮质激素，地塞米松，或盐皮质激素，醛固酮，调节量的Na，K-ATP酶位点的血管纹和螺旋韧带组织。手术切除肾上腺的雄性大鼠给予不同剂量的地塞米松或醛固酮7天，随后处死。用酶联免疫吸附试验定量测定血管纹和螺旋韧带匀浆的Na，K-ATP酶α亚基，使用显示与侧壁Na，K-ATP酶α亚基反应的单克隆抗体。血清地塞米松水平升高与血管纹和螺旋韧带Na，K-ATP酶α亚基水平显著升高相关（P
The objective of this study was to determine whether varying levels of either the synthetic glucocorticoid, dexamethasone, or the mineralocorticoid, aldosterone, modulate the quantity of Na,K-ATPase sites in stria vascularis and spiral ligament tissues. Surgically adrenalectomized male rats were administered different dosages of dexamethasone or aldosterone for 7 days and subsequently were sacrificed. Na,K-ATPase alpha-subunits of stria vascularis and spiral ligament homogenates were determined quantitatively by enzyme-linked immunosorbent assay, using a monoclonal antibody shown to react with lateral wall Na,K-ATPase alpha-subunit. Elevated serum levels of dexamethasone were correlated with significantly increased Na,K-ATPase alpha-subunit levels in both the stria vascularis and spiral ligament (P