Molecular cloning of rat klotho cDNA:: Markedly decreased expression of klotho by acute inflammatory stress
Molecular cloning of rat klotho cDNA:: Markedly decreased expression of klotho by acute inflammatory stress
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DOI：
10.1006/bbrc.1998.9576
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发表时间：
1998-10-29
影响因子：
3.1
通讯作者：
Nagail, R
中科院分区：
文献类型：
--
作者：
Ohyama, Y;Kurabayashi, M;Nagail, R
我们最近发现了一种新的基因，称为klotho，它参与抑制几种衰老表型，该基因编码一种膜蛋白，与细菌和植物的β-葡萄糖苷酶具有序列相似性。在这项研究中，我们分离了大鼠klotho cDNA并检查了其在大鼠体内的组织分布。推导的大鼠Klotho蛋白的氨基酸序列长度为1014个氨基酸，与小鼠和人Klotho蛋白的氨基酸序列同源性分别为94%和85%。使用大鼠klotho cDNA探针的北方印迹分析鉴定了主要在肾脏中表达的5.2 kb大小的单个转录物，而RT-PCR检测到脑、肺、肠和卵巢中也有低水平的表达。在发育过程中，klotho在肾脏中的表达在出生后显著增加。将大鼠klotho基因定位于12 q12，北方印迹分析显示，脂多糖（Lipopolysaccharide，LPS）可明显降低klotho基因的表达，提示急性炎症应激可影响klotho基因的表达。本研究导致更好地了解Klotho的生理和病理生理作用。(C)北京：科学出版社.
We have recently identified a novel gene, termed klotho that is involved in the suppression of several aging phenotypes, The gene encodes a membrane protein that shares sequence similarity with the p-glucosidases of bacteria and plants. In this study, we isolated rat klotho cDNA and examined its tissue distribution in rats. The deduced amino acid sequence of rat Klotho protein was 1014 amino acids in length and 94 and 85% homologous to those of mouse and human Klotho proteins, respectively. Northern blot analysis using the rat klotho cDNA probe identified a single transcript of 5.2 kb in size expressed predominantly in the kidney, while RT-PCR detected low levels of expression also in the brain, lung, intestine, and ovaries. During development, klotho expression in the kidney was markedly augmented after birth. Chromosomal localization of rat klotho was mapped to 12q12, Northern blot analysis showed that expression of klotho was markedly decreased by Lipopolysaccharide (LPS) in vivo suggesting that expression of klotho is affected by acute inflammatory stress. The present study leads to a better understanding of the physiologic and pathophysiologic roles Of Klotho. (C) 1998 Academic Press.