Identification of a cobia (Rachycentron canadum) CC chemokine gene and its involvement in the inflammatory response.
Identification of a cobia (Rachycentron canadum) CC chemokine gene and its involvement in the inflammatory response.
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DOI：
10.1016/j.fsi.2011.10.005
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发表时间：
2012
影响因子：
4.7
通讯作者：
Youlu Su;Zhixun Guo;Li-wen Xu;Jingzhe Jiang;Jiangyong Wang;Juan Feng
中科院分区：
文献类型：
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作者：
Youlu Su;Zhixun Guo;Li-wen Xu;Jingzhe Jiang;Jiangyong Wang;Juan Feng
趋化因子在炎症和生理条件下调节免疫细胞迁移。我们研究了军曹鱼CC趋化因子基因（RcCC 1）。全长RcCC 1 cDNA由673个核苷酸组成，编码已知CC趋化因子的典型的四个半胱氨酸排列的99个氨基酸的蛋白质。RcCC 1基因组DNA由3个外显子和2个内含子组成。系统发育分析表明RcCC 1与CC趋化因子MIP组关系最近。实时定量RT-PCR（qRT-PCR）分析显示RcCC 1在所有检测的组织中组成型表达，在鳃、血液、肾、脾和头肾中相对强表达。在头肾，脾脏和肝脏中的RcCC 1转录本在福尔马林灭活的弧菌carchariae（细菌疫苗）或多聚核糖肌苷酸多聚核糖胞苷酸（poly I：C）刺激后迅速上调。这些结果表明RcCC 1不仅在体内平衡中起作用，而且可能参与细菌和病毒感染的炎症反应。
The chemokines regulate immune cell migration under inflammatory and physiological conditions. We investigated a CC chemokine gene (RcCC1) from cobia (Rachycentron canadum). The full-length RcCC1 cDNA is comprised 673 nucleotides and encodes a four-cysteine arrangement 99-amino-acid protein typical of known CC chemokines. The genomic DNA of RcCC1 consists of three exons and two introns. Phylogenetic analysis showed that RcCC1 was closest to the MIP group of CC chemokines. Quantitative real-time RT-PCR (qRT-PCR) analysis revealed RcCC1 was constitutively expressed in all tissues examined, with relative strong expression in gill, blood, kidney, spleen, and head kidney. The RcCC1 transcripts in the head kidney, spleen, and liver were quickly up-regulated after stimulation with formalin-inactivated Vibrio carchariae (bacterial vaccine) or polyriboinosinic polyribocytidylic acid (poly I:C). These results indicate RcCC1 not only plays a role in homeostasis, but also may be involved in inflammatory responses to bacterial and viral infection.