Azaelectrocyclization on cell surface: convenient and general approach to chemical biology research
Azaelectrocyclization on cell surface: convenient and general approach to chemical biology research
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DOI：
10.1016/j.tet.2015.02.063
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发表时间：
2015-07-01
期刊：
影响因子：
2.1
通讯作者：
Tanaka, Katsunori
中科院分区：
文献类型：
--
作者：
Ogura, Akihiro;Tanaka, Katsunori
建立了方便通用的活细胞表面标记方法。通过应变促进的点击反应，含叠氮化物的荧光最初与含有二苯并环辛炔 (DIBO) 的不饱和酯醛缀合。然后用活细胞一锅法处理所得探针，在温和条件下通过氮杂电环化有效标记表面赖氨酸。 (C) 2015 Elsevier Ltd. 保留所有权利。
Convenient and general method for live cell surface labeling was established. Azide-containing fluorescences were initially conjugated with the unsaturated ester aldehyde containing the dibenzocyclooctyne (DIBO), by the strain-promoted click reaction. The resulting probes were then treated with the live cells in one-pot process, efficiently labeling the surface lysines by azaelectrocyclization under the mild conditions. (C) 2015 Elsevier Ltd. All rights reserved.