Lycopene production from glucose, fatty acid and waste cooking oil by metabolically engineered Escherichia coli
Lycopene production from glucose, fatty acid and waste cooking oil by metabolically engineered Escherichia coli
复制标题
DOI：
10.1016/j.bej.2020.107488
复制
发表时间：
2020-03-15
影响因子：
3.9
通讯作者：
Xie, Dongming
中科院分区：
文献类型：
--
作者：
Liu, Na;Liu, Bo;Xie, Dongming
番茄红素是一种抗氧化剂，可用作功能性食品或用于营养品、药物和化妆品应用的高价值产品。本论文首先在大肠杆菌中构建了甲羟戊酸和番茄红素的合成途径，以葡萄糖和甘油为亲水性底物合成番茄红素。进一步介绍了E. Coll.菌株导致从疏水底物如脂肪酸合成番茄红素的改进。在1 L补料分批反应器中，工程菌E. coll. FA 03-PM在与葡萄糖和水解废烹饪油（WCO）共进料的发酵中在40 h内产生2.7 g/L的番茄红素。这是第一个研究报告，高番茄红素生产发酵脂肪酸或废食用油。此外，生物量中番茄红素含量达到94 mg/g，这是迄今为止报道的工程E.大肠杆菌进行番茄红素的生产。研究成果还将有助于建立一个新的生物制造平台，从普通农产品，油脂或其废弃物中生产更多高价值产品。
Lycopene is an antioxidant that can be used as functional food or a high-value product for nutraceutical, pharmaceutical, and cosmetic applications. In this paper, the mevalonate and lycopene synthesis pathways were first built in Escherichia coll. to produce lycopene from hydrophilic substrates such as glucose and glycerol. Further introducing the fatty acid transport system in the E. coll. strain led to improved synthesis of lycopene from hydrophobic substrates such as fatty acids. In 1-L fed-batch bioreactors, the engineered E. coll. FA03-PM produced 2.7 g/L lycopene within 40 h in fermentation co-fed with glucose and hydrolyzed waste cooking oil (WCO). This is the first study that reports high lycopene production by fermentation with fatty acids or waste cooking oils. In addition, the lycopene content in biomass reached 94 mg/g, which is among the highest levels reported so far in engineered E. coli for lycopene production. The research results will also help build a new biomanufacturing platform for making more high-value products from the common agriculture commodities, oils and fats, or their wastes.