The mating projections of Saccharomyces cerevisiae and Candida albicans show key characteristics of hyphal growth
The mating projections of Saccharomyces cerevisiae and Candida albicans show key characteristics of hyphal growth
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DOI：
10.1016/j.funbio.2011.02.001
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发表时间：
2011-06-01
期刊：
影响因子：
2.5
通讯作者：
Sudbery, Peter
中科院分区：
文献类型：
--
作者：
Chapa-Y-Lazo, Bernardo;Lee, Sheu;Sudbery, Peter
真菌可以以多种生长形式生长：酵母、假菌丝和菌丝。人类真菌病原体白色念珠菌可以在这三种形式中生长。在这种真菌中，菌丝生长的特点是在菌丝顶端有一个类似Spitzenkorper的结构，在新外翻的芽管的底部周围有一条隔膜条带。萌芽酵母以酵母和假菌丝的形式生长，但通常不被认为是菌丝生长。我们在这里表明，在白色念珠菌和酿酒酵母的交配突起中，生长的顶端存在类似Spitzenkorper的结构，底部存在一条隔膜条带。此外，在酿酒酵母的交配投影中，Spa2和Bni1形成了Fm4-染色的三维球体的帽，与先前在白念珠菌菌丝中观察到的完全相同，这表明推测的Spitzenkorper可能是与极化体不同的结构。综上所述，这项工作表明，酿酒酵母和白色念珠菌的交配突起都显示出菌丝生长的关键特征。(C)2011年英国真菌学会。爱思唯尔有限公司出版。保留所有权利。
Fungi can grow in a variety of growth forms: yeast, pseudohyphae and hyphae. The human fungal pathogen Candida albicans can grow in all three of these forms. In this fungus, hyphal growth is distinguished by the presence of a Spitzenkorper-like structure at the hyphal tip and a band of septin bars around the base of newly evaginated germ tubes. The budding yeast Saccharomyces cerevisiae grows as yeast and pseudohyphae, but is not normally considered to show hyphal growth. We show here that in mating projections of both C. albicans and S. cerevisiae a Spitzenkorper-like structure is present at the growing tip and a band of septin bars is present at the base. Furthermore, in S. cerevisiae mating projections, Spa2 and Bni1 form a cap to the 3-dimensional ball of FM4-64 staining, exactly as previously observed in C. albicans hyphae, suggesting that the putative Spitzenkorper may be a distinct structure from the polarisome. Taken together this work shows that mating projections of both S. cerevisiae and C. albicans show the key characteristics of hyphal growth. (C) 2011 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.