Utility of urinary tubular markers for monitoring chronic tubulointerstitial injury after ischemia-reperfusion
Utility of urinary tubular markers for monitoring chronic tubulointerstitial injury after ischemia-reperfusion
复制标题
DOI：
10.1111/nep.12998
复制
发表时间：
2018-04-01
期刊：
影响因子：
2.5
通讯作者：
Shibagaki, Yugo
中科院分区：
文献类型：
--
作者：
Ichikawa, Daisuke;Kamijo-Ikemori, Atsuko;Shibagaki, Yugo
目的本研究的目的是阐明急性肾损伤（AKI）慢性期尿管标志物是否与慢性肾小管间质损伤相关。方法采用肾蒂钳夹10分钟或20分钟的方法，对雄性人L型脂肪酸结合蛋白（L-FABP）染色体转基因（Tg）小鼠进行缺血再灌注（I/R）损伤。在组织再灌注时进行对侧肾切除术。最后一次 I/R 后 20 天对肾脏进行分析。结果 I/R 后 20 天，20 分钟 I/R 组的血清肌酐水平显着高于 10 分钟 I/R 组和对照组，并且 10 分钟 I/R 组和对照组之间相似。 I/R后20天的肾小管间质损伤程度，20分钟I/R组比10分钟I/R组和对照组显着更严重，10分钟I/R组也比对照组更严重。 I/R后20天尿中人L-FABP、白蛋白和肾损伤分子1（KIM-1）的水平在20分钟I/R中显着高于对照组，而尿L-FABP在10分钟I/R中显着高于对照组。相反，尿中性粒细胞明胶酶相关脂质运载蛋白水平在三组之间没有显着差异。最后，I/R后20天的尿液中人L-FABP、白蛋白和KIM-1水平与肾损伤程度显着相关。结论在临床实践中，人L-FABP、白蛋白和KIM-1的尿液水平可能有助于监测AKI向CKD的转变。
AimThe aim of this study was to elucidate whether urinary tubular markers during the chronic phase of acute kidney injury (AKI) are associated with chronic tubulointerstitial damage.MethodsMale human L-type fatty acid binding protein (L-FABP) chromosomal transgenic (Tg) mice underwent ischaemic reperfusion (I/R) injury via renal pedicle clamping for either 10min or 20min. Contralateral nephrectomy was performed at the time of tissue reperfusion. The kidneys were analyzed 20days after the last I/R.ResultsSerum creatinine levels 20days post-I/R were significantly higher in the 20min I/R than in the 10min I/R and control groups and were similar between the 10min I/R and control groups. The degree of tubulointerstitial damage 20days post-I/R was significantly more severe in the 20min I/R than in the 10min I/R and control groups, as well as in the 10min I/R than in the control group. Urinary levels of human L-FABP, albumin, and kidney injury molecule-1 (KIM-1) 20days post-I/R were significantly higher in the 20min I/R than in the control group, whereas urinary L-FABP was significantly higher in the 10min I/R than in the control group. Conversely, urinary neutrophil gelatinase-associated lipocalin levels did not significantly differ between the three groups. Finally, the urinary levels of human L-FABP, albumin, and KIM-1 levels 20days post-I/R were significantly correlated with the degree of renal damage.ConclusionsUrinary levels of human L-FABP, albumin and, KIM-1 may be useful for monitoring AKI-to-CKD transition in clinical practice.