Human papillomavirus types 16 and 18 infection in infiltrating adenocarcinoma of the cervix: PCR analysis of 138 cases and correlation with histologic type and grade.
Human papillomavirus types 16 and 18 infection in infiltrating adenocarcinoma of the cervix: PCR analysis of 138 cases and correlation with histologic type and grade.
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DOI：
10.1093/ajcp/106.1.52
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发表时间：
1996-07
影响因子：
3.5
通讯作者：
P. Tenti;Solange Romagnoli;Enrico Silini;R. Zappatore;Arsenio Spinillo;Paolo Giunta;Anna Cappellini;N. Vesentini;C. Zara;Luciano Carnevali
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文献类型：
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作者：
P. Tenti;Solange Romagnoli;Enrico Silini;R. Zappatore;Arsenio Spinillo;Paolo Giunta;Anna Cappellini;N. Vesentini;C. Zara;Luciano Carnevali
作者通过 PCR 138 个浸润性宫颈腺癌（27 个 1 级、76 个 2 级和 35 个 3 级）调查了人乳头瘤病毒 (HPV) 16 和 18 感染的存在情况。其中包括 95 个（68.8%）粘液性肿瘤和 43 个（31.2%）非粘液性肿瘤。 HPV感染总体患病率为84.8%； 28.3% 的病例 HPV 16 呈阳性，29.7% 的病例呈 HPV 18 阳性，26.8% 的病例两种 HPV 均呈阳性。使用共有引物 MY09/MY11 扩增 HPV 16 和 18 阴性病例未能产生任何具有 HPV DNA 序列的其他肿瘤。 HPV 感染患者比 HPV 阴性患者年轻 (P = .001)。 HPV 类型与年龄无关。 95.8% 的粘液性肿瘤和 60.5% 的非粘液性肿瘤中发现人乳头瘤病毒感染 (P < .001)，在 1、2 和 3 级腺癌中分布均匀。我们的研究结果证实了 HPV 16 和 18 在宫颈腺癌发展中的关键作用，特别是在粘液组织型中。不存在 HPV 感染、患者年龄较大和非粘液分化可能会鉴定出具有不同发病机制的宫颈腺癌亚型。
The authors investigated by PCR 138 infiltrating cervical adenocarcinoma (27 grade 1, 76 grade 2, and 35 grade 3) for the presence of human papillomavirus (HPV) 16 and 18 infection. They included 95 (68.8%) mucinous and 43(31.2%) non-mucinous tumors. The overall prevalence of HPV infection was 84.8%; 28.3% of the cases were positive for HPV 16, 29.7% for HPV 18, and 26.8% for both HPVs. Amplification of HPV 16 and 18 negative cases with consensus primers MY09/MY11 failed to yield any additional tumors with HPV DNA sequences. Patients with HPV infection were younger than the patients who were HPV-negative (P = .001). The type of HPV was unrelated to age. Human papillomavirus infection was found in 95.8% mucinous and in 60.5% non-mucinous tumors (P < .001), with even distribution among grade 1, 2 and 3 adenocarcinoma. Our findings confirm the key role of HPV 16 and 18 in the development of cervical adenocarcinoma, particularly in mucinous histotypes. The absence of HPV infection, the old age of patients and the non-mucinous differentiation may identify a subset of cervical adenocarcinoma with different etiopathogenesis.