Diversity and evolution of the Cydia pomonella granulovirus
Diversity and evolution of the Cydia pomonella granulovirus
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DOI：
10.1099/vir.0.006999-0
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发表时间：
2009-03-01
影响因子：
3.8
通讯作者：
Jehle, Johannes A.
中科院分区：
文献类型：
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作者：
Eberle, Karolin E.;Sayed, Samy;Jehle, Johannes A.
对来自伊朗和格鲁吉亚的8株苹果蠹蛾颗粒体病毒（Cydia pomonella granulovirus，CpGV）和1株英国CpGV分离株进行了限制性片段长度多态性（RFLP）分析和部分基因组扩增及测序。根据观察到的RFLPs，这些分离株的大多数优势基因型可以分配到那些在以前发现的分离株来自墨西哥（CpGV-M），英国（CpGV-E）和俄罗斯（CpGV-R）。我们建议将这些分离株分别命名为基因组A、B和C型。在三个分离株中鉴定出第四种基因组类型，并将其指定为D型。具有A、B和D型基因组的菌株含有C型基因组中不存在的4个开放阅读框（ORF 63-CRF 66）。在其他颗粒病毒中缺乏这些ORF表明C型基因组在进化上是其他基因组类型的祖先。B和D型基因组含有额外插入的0.7 kb的非蛋白质编码区，其位于不同的基因组位置。通过对晚表达因子8（lef-8）、lef-9和多角体蛋白/颗粒蛋白（polh/poln）基因部分序列的分析，发现了与RFLP类型相对应的单核苷酸多态性（SNP）。基于这些SNPs的系统发育分析证实了C型基因组的祖先。C型病毒对苹果蠹蛾幼虫的毒力也低于其他病毒型。总之，已知的CpGV分离株的多样性可以用四种主要的基因组类型来描述，它们似乎以基因型混合物的形式存在于不同的分离株中。
Eight new field isolates of Cydia pomonella granulovirus (CpGV) originating in Iran and Georgia and one English CpGV isolate were analysed for restriction fragment length polymorphisms (RFLPs) and by partial genome amplification and sequencing. According to the observed RFLPs, most of the predominant genotypes of these isolates could be assigned to those present in previously found isolates originating from Mexico (CpGV-M), England (CpGV-E) and Russia (CpGV-R). We suggest that these isolates should be designated genome A, B and C types, respectively. A fourth genome type was identified in three isolates and is designated D type. The isolates with A, B and D type genomes contained four open reading frames (ORFs) (ORF63-CRF66) not present in C type genomes. The lack of these ORFs in other granuloviruses suggests that the C type genome is evolutionarily ancestral to the other genome types. The B and D type genomes contained an additional insertion of a non-protein coding region of 0.7 kb, which was at different genome locations. Analysis of the partial gene sequences of late expression factor 8 (lef-8), lef-9 and polyhedrin/granulin (polh/gran) genes revealed single nucleotide polymorphisms (SNPs) that corresponded to the RFLP types. Phylogenetic analyses based on these SNPs corroborated the proposed ancestry of the C type genome. C type viruses were also less virulent to neonate codling moth larvae than the other virus types. In conclusion, the known diversity of CpGV isolates can be described by four major genome types, which appear to exist in different isolates as genotype mixtures.