Accumulation of oligoclonal T cells in the infiltrating lymphocytes in oral lichen planus
Accumulation of oligoclonal T cells in the infiltrating lymphocytes in oral lichen planus
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DOI：
10.1034/j.1600-0714.2003.00143.x
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发表时间：
2003-05-01
影响因子：
3.3
通讯作者：
Shirasuna, K
中科院分区：
文献类型：
--
作者：
Kawamura, E;Nakamura, S;Shirasuna, K
背景资料：口腔扁平苔藓（oral lichen planus，OLP）中特异性T细胞受体（T cell receptor，TCR）的鉴定是揭示T细胞识别的致病性抗原，进而阐明OLP发病机制和病因的重要步骤之一。在7名OLP患者的颊粘膜活检标本和外周血单核细胞（PBMC）中，结果：Vbeta家族在活检标本中的表达具有明显的异质性，但与PBMC中观察到的Vbeta家族相比，它们受到限制。与PBMC相比，主要在活检标本中表达的Vbeta家族在个体患者中仍然是异质的，并且在患者之间存在差异;然而，Vbeta2、Vbeta6和Vbeta19通常在超过一半的患者的活检标本中占主导地位。在主要在活检标本中表达的Vbeta家族中，在包括Vbeta 6和Vbeta 19的大多数Vbeta家族中观察到T细胞克隆型的积累;然而，在包括Vbeta 2的少数Vbeta家族中未观察到T细胞克隆型的积累。这些结果表明，携带V β 2，V β 6，或V β 19基因产物倾向于在OLP损伤中扩增，这是由于用大多数V β家族的MHC分子上的名义抗原的限制性表位原位刺激的结果，即使仅在少数群体中，或少数V β家族的共同超抗原。
Background: Identification of a disease-specific and possibly pathogenic T-cell receptor (TCR) in oral lichen planus (OLP) is one of the most important steps to reveal the pathogenic antigen recognized by the T cells and thereby elucidate the pathogenesis and etiology of OLP.Methods: In buccal mucosa biopsy specimens and peripheral blood mononuclear cells (PBMC) from seven patients with OLP, the TCR Vbeta gene usage was examined by polymerase chain reaction-based and single-strand conformation polymorphism analyses.Results: The Vbeta families expressed in the biopsy specimens were markedly heterogeneous, but they were restricted in comparison to those observed in the PBMC. The Vbeta families predominantly expressed in the biopsy specimens in comparison with the PBMC were still heterogeneous in individual patients and differed from patient to patient; however, Vbeta2, Vbeta6, and Vbeta19 were commonly predominant in the biopsy specimens from more than half of the patients. Among the Vbeta families predominantly expressed in the biopsy specimens, the accumulation of T-cell clonotypes was observed in the majority of the Vbeta families including Vbeta6 and Vbeta19; however, it was not observed in the minority of the Vbeta families including Vbeta2.Conclusions: These results suggest that unique T-cell populations bearing Vbeta2, Vbeta6, or Vbeta19 gene products tend to expand in OLP lesions as a consequence of in situ stimulation with a restricted epitope of either a nominal antigen on the MHC molecule for the majority of the Vbeta families, even if only in minor populations, or of a common superantigen for the minority of the Vbeta families.