Contents of caffeoylquinic acid compounds in the storage roots of sixteen sweetpotato genotypes and their potetial biological activity
Contents of caffeoylquinic acid compounds in the storage roots of sixteen sweetpotato genotypes and their potetial biological activity
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DOI：
10.21273/jashs.133.4.492
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发表时间：
2008-07-01
影响因子：
1.9
通讯作者：
Snook, Maurice E.
中科院分区：
文献类型：
--
作者：
Harrison, Howard F., Jr.;Mitchell, Trevor R.;Snook, Maurice E.
咖啡酰奎宁酸化合物广泛存在于植物中。它们保护植物免受捕食和感染，并可能在人类饮食中具有多种有益功能。研究了16个甘薯品种[Ipomoea batatas（L.）Lam.]确定基因型。在不同基因型中，这4种化合物的含量以皮层最高，中柱次之，周壁最低。在不同基因型中，周皮中绿原酸含量为16 ~ 212 μ g·g ~（-1），皮层中绿原酸含量为826 ~ 7274 μ g·g ~（-1），中柱中绿原酸含量为171 ~ 4326 μ g·g ~（-1）。3，5-DCQA异构体占总DCQA的80%以上。在大多数基因型中，皮层和中柱组织中3，5-DCQA和绿原酸含量相近，但周鞘组织中绿原酸含量低于3，5-DCQA。在16个基因型中，周鞘中DCQA总含量为0 ~ 1775 μ g·g（-1），皮层中DCQA总含量为883 ~ 8764 μ g·g（-1），中柱中DCQA总含量为187 ~ 4768 μ g·g（-1）。在一个小的种质资源收集中发现的巨大差异表明，选择或育种高咖啡酰奎尼酸含量的甘薯基因型是可能的。四种咖啡酰奎尼酸化合物占甘薯近缘种大根牵牛[Ipomoea pandurata（L.）G.F. W Meyer]，表明它可能是化合物的良好来源。从甘薯和L pandurata组织中分离的DCQA以及咖啡酸和绿原酸标准品的效果在Proso小米（Panicum milliaceum L.）茄病镰刀菌Fusarium solani（Sace.）马特，和细菌生长生物测定。咖啡酸、绿原酸和3，5-DCQA在小米和F. solani生物测定，但3，5-DCQA是细菌生长生物测定中抑制作用最小的化合物。它们的生物活性表明，咖啡酰奎宁酸化合物有助于一些甘薯无性系的化感潜力和对根部疾病的抗性。
Caffeoylquinic acid compounds are widespread in plants. They protect plants against predation and infection and may have several beneficial functions in the human diet. The contents of chlorogenic acid and the 3,4-, 3,5-, and 4,5- isomers of dicaffeoylquinic acid (DCQA) in the storage root tissues of 16 sweetpotato [Ipomoea batatas (L.) Lam.] genotypes were determined. Averaged over genotypes, the contents of the four compounds were highest in the cortex, intermediate in the stele, and lowest in the periderm. Among the genotypes, chlorogenic acid contents ranged from 16 to 212 mu g.g(-1) in periderm, from 826 to 7274 jig-g-in cortex, and from 171 to 4326 mu g.g(-1) in stele. The 3,5-DCQA isomer comprised over 80% of total DCQA. In most genotypes, 3,5-DCQA and chlorogenic acid contents were similar in cortex and stele tissues, but chlorogenic acid was lower than 3,5-DCQA in periderm tissue. Among the 16 genotypes, total DCQA contents ranged from 0 to 1775 mu g.g(-1) dry weight in periderm, from 883 to 8764 mu g.g(-1) in cortex, and from 187 to 4768 mu g-g(-1) in stele. The large differences found in a small germplasm collection suggest that selecting or breeding sweetpotato genotypes with high caffeoylquinic acid content is possible. The four caffeoylquinic acid compounds comprised over 3% of the dry weight of storage roots of the sweetpotato relative, bigroot morningglory [Ipomoea pandurata (L.) G.F.W. Meyer], indicating that it may be a good source for the compounds. The effect of DCQAs isolated from sweetpotato and L pandurata tissue and caffeic and chlorogenic acid standards were tested in proso millet (Panicum milliaceum L.), Fusarium solani (Sace.) Mart., and bacterial growth bioassays. Caffeic acid, chlorogenic acid, and 3,5-DCQA were most inhibitory in millet and F. solani bioassays, but 3,5-DCQA was the least inhibitory compound in bacterial growth bioassays. Their activity in the bioassays suggests that the caffeoylquinic acid compounds contribute to the allelopathic potential and resistance to root diseases of some sweetpotato clones.