Spontaneous calcium transients are required for neuronal differentiation of murine neural crest
Spontaneous calcium transients are required for neuronal differentiation of murine neural crest
复制标题
DOI：
10.1006/dbio.1999.9433
复制
发表时间：
1999-11-15
影响因子：
2.7
通讯作者：
Matsumoto, SG
中科院分区：
文献类型：
--
作者：
Carey, MB;Matsumoto, SG
我们已经表明，培养的小鼠神经嵴（NC）细胞表现出短暂的细胞内钙离子的增加。高达50%的培养的NC衍生细胞在神经元分化期间表现出钙瞬变。随着神经活性的下降，活性NC衍生细胞的百分比和它们的钙峰频率也下降。钙瞬时活性的降低与对硫柳汞敏感性的降低相关，硫柳汞可使肌醇1，4，5-三磷酸受体敏感。Thimerectin增加了活跃的NC衍生细胞中的振荡频率，并在静止细胞的亚群中诱导它们。随着神经发生的结束，NC衍生的细胞变得对硫柳汞无反应。使用时间依赖性神经元性状的表达，我们确定，神经元表现出自发的钙瞬变早神经元表型可以检测到，并继续通过收购咖啡因的敏感性，不久之后，钙瞬变活动停止。一个亚群的非神经元NC衍生细胞表现出钙瞬变活动在同一时间框架内的神经发生的文化。暴露于20 mM Mg 2+的NC衍生细胞阻断钙瞬变活性，减少神经元数量，而不影响分化的神经元的存活。使用谱系追踪分析，我们发现50%的活性NC衍生细胞产生含有神经元的克隆，而非活性细胞没有。我们推测，钙瞬变活动建立了未分化的NC细胞的神经元的能力。(C)北京：科学出版社.
We have shown that cultured mouse neural crest (NC) cells exhibit transient increases in intracellular calcium. Up to 50% of the cultured NC-derived cells exhibited calcium transients during the period of neuronal differentiation. As neurogenic activity declined, so did the percentage of active NC-derived cells and their calcium spiking frequency. The decrease in calcium transient activity correlated with a decreased sensitivity to thimerosal, which sensitizes inositol 1,4,5-triphosphate receptors. Thimerosal increased the frequency of oscillations in active NC-derived cells and induced them in a subpopulation of quiescent cells. As neurogenesis ended, NC-derived cells became nonresponsive to thimerosal. Using the expression of time-dependent neuronal traits, we determined that neurons exhibited spontaneous calcium transients as early as a neuronal phenotype could be detected and continued through the acquisition of caffeine sensitivity, soon after which calcium transient activity stopped. A subpopulation of nonneuronal NC-derived cells exhibited calcium transient activity within the same time frame as neurogenesis in culture. Exposing NC-derived cells to 20 mM Mg2+ blocked calcium transient activity and reduced neuronal number without affecting the survival of differentiated neurons. Using lineage-tracing analysis, we found that 50% of active NC-derived cells gave rise to clones containing neurons, while inactive cells did not. We hypothesize that calcium transient activity establishes a neuronal competence for undifferentiated NC cells. (C) 1999 Academic Press.