CHARACTERIZATION OF SYMBIONIN WITH ANTISYMBIONIN ANTISERUM
CHARACTERIZATION OF SYMBIONIN WITH ANTISYMBIONIN ANTISERUM
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DOI：
10.1016/0020-1790(90)90064-2
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发表时间：
1990-01-01
期刊：
影响因子：
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通讯作者：
ISHIKAWA, H
中科院分区：
文献类型：
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作者：
HARA, E;FUKATSU, T;ISHIKAWA, H
将从豌豆蚜虫中提纯的共生素注射到兔体内，获得了抗共生素抗血清。在免疫印迹中，除了共生素，许多较小的蛋白质与抗血清发生交叉反应，这表明共生素在蚜虫组织中很容易被降解。当用抗共生素抗血清进行免疫印迹时，共生型和非共生型蚜虫的单位湿重中的共生素含量都随着年龄的增长而降低。在分子质量和免疫原性方面，五种不同种类的蚜虫具有与共生素相关的蛋白质类别。用抗共生素抗血清对蚜虫组织进行免疫组织化学研究表明，共生素仅定位于含有主要共生体的真菌细胞中。
Anti-symbionin antiserum was obtained by injecting symbionin purified from pea aphids into a rabbit. On immunoblotting, in addition to symbionin many smaller proteins cross-reacted with the antiserum suggesting that symbionin in the aphid tissue is prone to degradation. When estimated by immunoblotting with anti-symbionin antiserum, the symbionin content per unit wet weight of both symbiotic and aposymbiotic aphids was shown to decrease with age. Five distinct aphid species were shown to share a protein class related to symbionin in terms of molecular mass and immunogenicity. Immuno-histochemistry of aphid tissues with anti-symbionin antiserum indicated that symbionin is localized exclusively within the mycetocyte harboring the primary symbionts.