Mediator release in an isolated airway segment in subjects with asthma.
Mediator release in an isolated airway segment in subjects with asthma.
复制标题
哮喘受试者隔离气道段的介质释放。
DOI：
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复制
发表时间：
1988
期刊：
影响因子：
--
通讯作者：
W. Eschenbacher
中科院分区：
文献类型：
--
作者：
T. Gravelyn;P. M. Pan;W. Eschenbacher
为了确定暴露于高渗刺激后人类受试者的气道中是否会发生介质释放，我们使用专门设计的双球囊导管用900 mosmol氯化钠在体内挑战分离的气道段。为了确定介质浓度的差异是否与气道反应性或特应性相关，我们研究了3个独立的组：6名特应性哮喘受试者; 6名特应性非哮喘受试者;和6名非特应性非哮喘正常受试者。此外，为了确定导管本身的存在是否是一种刺激，我们研究了6名特应性哮喘受试者暴露于等渗氯化钠后（假暴露）。通过放射免疫测定法测定灌洗液标本中的PGD 2、PGF 2 α、PGE 2、TxB 2、LTC 4和LTB 4，并通过改良的单同位素酶法测定组胺。哮喘受试者的基线灌洗样本（支气管肺泡灌洗和隔离气道段灌洗）的组胺浓度显著高于特应性或正常受试者（p <0.05）。灌洗液中组胺浓度与受试者气道反应性呈显著负相关（r =-0.53，p <0.0001）。哮喘受试者所有灌洗液中组胺、PGD 2、PGF 2 α和TxB 2浓度之间存在显著相关性（所有比较的p <0.03，总体一致性的p <0.0001）。作为高渗激发的结果，哮喘受试者中组胺、PGD 2和PGF 2 α的浓度显著增加。（250字处删节）
To determine if mediator release would occur in the airways of human subjects after exposure to a hyperosmolar stimulus, we challenged isolated airway segments in vivo with 900 mosmol sodium chloride using a specially designed double-balloon catheter. In order to determine if differences in mediator concentrations correlated with airway responsiveness or with atopy, we studied 3 separate groups: 6 atopic, asthmatic subjects; 6 atopic, nonasthmatic subjects; and 6 nonatopic, nonasthmatic normal subjects. In addition, to determine if the presence of the catheter itself was a stimulus, we studied 6 atopic, asthmatic subjects after exposure to isotonic sodium chloride alone (sham exposure). Lavage specimens were assayed for PGD2, PGF2 alpha, PGE2, TxB2, LTC4, and LTB4 by radioimmunoassay and for histamine by the modified single isotope enzymatic assay. The histamine concentration of the baseline lavage samples (bronchoalveolar lavage and isolated airway segment lavage) for the subjects with asthma were significantly greater than for the atopic or normal subjects (p less than 0.05). There was a significant negative correlation between histamine concentration in the lavage samples and the airway responsiveness of the subjects (r = -0.53, p less than 0.0001). There was a marked correlation among the concentrations of histamine, PGD2, PGF2 alpha, and TxB2 in all the lavage fluids for the subjects with asthma (p less than 0.03 for all comparisons and p less than 0.0001 for overall concordance). As a result of hyperosmolar challenge, there was a significant increase in the concentration of histamine, PGD2, and PGF2 alpha in the subjects with asthma.(ABSTRACT TRUNCATED AT 250 WORDS)
DOI：
10.1164/arrd.1987.135.5.1043
发表时间：
2015-05
期刊：
The American review of respiratory disease
影响因子：
--
作者：
P. Eggleston;A. Kagey-sobotka;L. Lichtenstein
通讯作者：
P. Eggleston;A. Kagey-sobotka;L. Lichtenstein
影响因子：
4.4
作者：
R. Lewis;N. Soter;P. Diamond;K. Austen;J. Oates;L. Roberts
通讯作者：
R. Lewis;N. Soter;P. Diamond;K. Austen;J. Oates;L. Roberts
影响因子：
15.9
作者：
TOGIAS, AG;NACLERIO, RM;LICHTENSTEIN, LM
通讯作者：
LICHTENSTEIN, LM