Genomic characterization of a novel poxvirus from a flying fox: evidence for a new genus?
Genomic characterization of a novel poxvirus from a flying fox: evidence for a new genus?
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DOI：
10.1099/jgv.0.000538
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发表时间：
2016-09-01
影响因子：
3.8
通讯作者：
Upton, Chris
中科院分区：
文献类型：
--
作者：
O'Dea, Mark A.;Tu, Shin-Lin;Upton, Chris
一只澳大利亚小红狐蝠（Pteropus scapulatus）的尸体在围栏上被诱捕后死亡，被提交给实验室进行澳大利亚蝙蝠狂犬病病毒排除测试，结果为阴性。在死后，注意到多个结节上的翅膀膜，因此，简并PCR引物靶向痘病毒DNA聚合酶基因被用来筛选痘病毒。痘病毒PCR筛选是阳性的，PCR产物的测序显示与痘病毒科成员的DNA聚合酶基因的相似性非常低，但显著。从病变中提取的DNA的下一代测序返回了132 353个核苷酸（nt）的重叠群，其进一步延伸以产生133 492 nt的近全长病毒基因组。基因组分析显示，它是AT丰富的反向末端重复序列至少1314 nt，并含有143个预测的基因。该基因组包含了令人惊讶的大量（29）的基因没有发现在其他痘病毒，其中之一似乎是一个同源的哺乳动物肿瘤坏死因子相关的凋亡诱导配体（TRAIL）基因。系统发育分析表明，这里描述的痘病毒与从蝙蝠或其他物种中分离的任何其他痘病毒没有密切关系，并且它可能应该被置于一个新的属中。
The carcass of an Australian little red flying fox (Pteropus scapulatus) which died following entrapment on a fence was submitted to the laboratory for Australian bat lyssavirus exclusion testing, which was negative. During post-mortem, multiple nodules were noted on the wing membranes, and therefore degenerate PCR primers targeting the poxvirus DNA polymerase gene were used to screen for poxviruses. The poxvirus PCR screen was positive and sequencing of the PCR product demonstrated very low, but significant, similarity with the DNA polymerase gene from members of the Poxviridae family. Next-generation sequencing of DNA extracted from the lesions returned a contig of 132 353 nucleotides (nt), which was further extended to produce a near full-length viral genome of 133 492 nt. Analysis of the genome revealed it to be AT-rich with inverted terminal repeats of at least 1314 nt and to contain 143 predicted genes. The genome contains a surprisingly large number (29) of genes not found in other poxviruses, one of which appears to be a homologue of the mammalian TNF-related apoptosis-inducing ligand (TRAIL) gene. Phylogenetic analysis indicates that the poxvirus described here is not closely related to any other poxvirus isolated from bats or other species, and that it likely should be placed in a new genus.