Molecularly imprinted polymer anchored on the surface of denatured bovine serum albumin modified CdTe quantum dots as fluorescent artificial receptor for recognition of target protein.
Molecularly imprinted polymer anchored on the surface of denatured bovine serum albumin modified CdTe quantum dots as fluorescent artificial receptor for recognition of target protein.
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DOI：
10.1016/j.bios.2011.09.042
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发表时间：
2012-01
影响因子：
12.6
通讯作者：
Wei Zhang;Xiwen He;Yang Chen;Wen-You Li;Yukui Zhang
中科院分区：
文献类型：
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作者：
Wei Zhang;Xiwen He;Yang Chen;Wen-You Li;Yukui Zhang
采用表面分子印迹技术，将分子印迹聚合物（MIP）锚定在变性牛血清白蛋白（dBSA）修饰的CdTe量子点（QDs）表面，制备了一种新型的基于MIP的荧光人工受体。该方法结合了分子印迹技术的优点和CdTe量子点的荧光特性。dBSA不仅可以修饰CdTe量子点的表面缺陷，而且可以作为辅助单体来创建有效的识别位点。以溶菌酶（Lyz）、细胞色素c（Cyt）和甲基化牛血清白蛋白（mBSA）三种蛋白质为模板分子，采用溶胶-凝胶法（印迹法）合成受体。荧光和结合实验结果表明，受体对相应的模板识别性能。在最佳条件下，Lyz的线性范围为1.4×10− 8 ~ 8.5×10− 6 M，检出限为6.8nM。并将其应用于真实的样品中Lyz的分离检测。该荧光人工受体可作为设计高效人工荧光受体识别靶蛋白的起点。
A new type of molecularly imprinted polymer (MIP)-based fluorescent artificial receptor was developed by anchoring MIP on the surface of denatured bovine serum albumin (dBSA) modified CdTe quantum dots (QDs) using the surface molecular imprinting process. The approach combined the merits of molecular imprinting technology and the fluorescent property of the CdTe QDs. The dBSA was used not only to modify the surface defects of the CdTe QDs, but also as assistant monomer to create effective recognition sites. Three different proteins, namely lysozyme (Lyz), cytochrome c (Cyt) and methylated bovine serum albumin (mBSA), were tested as the template molecules and then the receptors were synthesized by sol–gel reaction (imprinting process). The results of fluorescence and binding experiments demonstrated the recognition performance of the receptors toward the corresponding template. Under optimum conditions, the linear range for Lyz was from 1.4×10−8to 8.5×10−6M, and the detection limit was 6.8nM. Moreover, the new artificial receptors were applied to separate and detect Lyz in real samples. This fluorescent artificial receptor may serve as a starting point in the design of highly effective synthetic fluorescent receptor for recognition of target protein.