COLD SHOCK DOMAIN PROTEIN 3 is involved in salt and drought stress tolerance in Arabidopsis.
COLD SHOCK DOMAIN PROTEIN 3 is involved in salt and drought stress tolerance in Arabidopsis.
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DOI：
10.1016/j.fob.2013.10.003
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发表时间：
2013
期刊：
影响因子：
2.6
通讯作者：
Imai R
中科院分区：
文献类型：
--
作者：
Kim MH;Sato S;Sasaki K;Saburi W;Matsui H;Imai R
冷休克蛋白（CSP）是细菌对冷的反应，作为RNA分子伴侣在冷适应中起重要作用。拟南芥冷休克结构域蛋白3（AtCSP 3）与细菌CSPs共享一个结构域，并参与获得抗冻性。我们之前的研究表明，AtCSP 3敲除突变体（atcsp 3 -2）中下调的许多基因在功能上与盐和干旱以及寒冷的反应相关。在这里，我们研究了AtCSP 3在盐和干旱胁迫耐受性的参与。我们发现AtCSP 3在盐和干旱胁迫下被诱导，并受到阿坝的调节。AtCSP 3基因敲除突变体（atcsp 3 -2）在盐胁迫和干旱胁迫下的存活率较低。相反，AtCSP 3-过表达的植物表现出较高的存活率后，这些压力处理。大多数的基因被下调的atcsp 3 -2突变体被发现是可诱导的盐和干旱胁迫后，和上调的AtCSP 3-overexpressors。总之，我们的数据表明，AtCSP 3参与拟南芥盐和干旱胁迫耐受性的调节。拟南芥冷休克结构域蛋白3（AtCSP 3）在盐和干旱胁迫期间被诱导。AtCSP 3基因敲除突变体在盐胁迫和干旱胁迫下的存活率较低。AtCSP 3过表达的植物在盐和干旱胁迫后表现出更高的存活率。AtCSP 3参与拟南芥盐和干旱胁迫耐受性的调节。
Cold shock proteins (CSPs) of bacteria are produced in response to cold and function as RNA chaperones that are essential for cold adaptation. Arabidopsis thaliana COLD SHOCK DOMAIN PROTEIN 3 (AtCSP3) shares a domain with bacterial CSPs and is involved in acquisition of freezing tolerance. Our previous study revealed that many of the genes that are down regulated in an AtCSP3 knockout mutant (atcsp3–2) are functionally associated with responses to salt and drought as well as cold. Here, we examined the involvement of AtCSP3 in salt and drought stress tolerance. We found that AtCSP3 is induced during salt and drought stresses, and is regulated by ABA. A knockout mutant of AtCSP3 (atcsp3–2) showed lower survival rates after salt and drought stress treatments. Conversely, the AtCSP3-overexpressing plants displayed higher survival rates after treatment with these stresses. Most of the genes that were down regulated in the atcsp3–2 mutant were found to be inducible upon salt and drought stresses, and upregulated in the AtCSP3-overexpressors. Together, our data demonstrates that AtCSP3 is involved in the regulation of salt and drought stress tolerance in Arabidopsis. Arabidopsis thaliana COLD SHOCK DOMAIN PROTEIN 3 (AtCSP3) is induced during salt and drought stresses. A knockout mutant of AtCSP3 showed lower survival rates after salt and drought stresses. AtCSP3-overexpressing plants displayed higher survival rates after salt and drought stresses. AtCSP3 is involved in the regulation of salt and drought stress tolerance in Arabidopsis.