A glucose-starvation response governs endocytic trafficking and eisosomal retention of surface cargoes in budding yeast.
A glucose-starvation response governs endocytic trafficking and eisosomal retention of surface cargoes in budding yeast.
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葡萄糖饥饿反应控制着出芽酵母的内吞运输和表面货物的泌体保留。
DOI：
10.1242/jcs.257733
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发表时间：
2021-01-25
影响因子：
4
通讯作者：
MacDonald C
中科院分区：
文献类型：
--
作者：
Laidlaw KME;Bisinski DD;Shashkova S;Paine KM;Veillon MA;Leake MC;MacDonald C
真核细胞使其新陈代谢适应细胞外环境。对营养胁迫反应的表面货物蛋白的下调减少了合成代谢过程的负担，同时提高了溶酶体中分解代谢的产量。我们表明，酵母中的葡萄糖饥饿触发了一种转录反应，增加了质膜的内化。葡萄糖饥饿反应中Mig1转录抑制子的核出口增加了Yap1801和Yap1802网状蛋白适配器的水平，这足以增加货物的内化。除此之外，我们还发现葡萄糖饥饿会导致不依赖于Mig1的各种常染色体因子的转录上调。这些因素通过Ygr130c的存在以及Pil1的生化和生物物理变化，将部分营养转运体隔离在现有的Eisosome上，使细胞能够在整个饥饿期持续存在，并在恢复到充足的条件时最大限度地吸收养分。这为细胞从葡萄糖饥饿中迅速恢复提供了生理上的好处。总体而言，在葡萄糖饥饿期间，这种表面蛋白质景观的重塑将代谢调整为可用营养素。摘要：许多细胞表面蛋白的内吞作用是在葡萄糖饥饿后触发的，饥饿后的恢复是由保存在Eisosome表面的一小部分营养转运蛋白介导的。
Eukaryotic cells adapt their metabolism to the extracellular environment. Downregulation of surface cargo proteins in response to nutrient stress reduces the burden of anabolic processes whilst elevating catabolic production in the lysosome. We show that glucose starvation in yeast triggers a transcriptional response that increases internalisation from the plasma membrane. Nuclear export of the Mig1 transcriptional repressor in response to glucose starvation increases levels of the Yap1801 and Yap1802 clathrin adaptors, which is sufficient to increase cargo internalisation. Beyond this, we show that glucose starvation results in Mig1-independent transcriptional upregulation of various eisosomal factors. These factors serve to sequester a portion of nutrient transporters at existing eisosomes, through the presence of Ygr130c and biochemical and biophysical changes in Pil1, allowing cells to persist throughout the starvation period and maximise nutrient uptake upon return to replete conditions. This provides a physiological benefit for cells to rapidly recover from glucose starvation. Collectively, this remodelling of the surface protein landscape during glucose starvation calibrates metabolism to available nutrients. Summary: Endocytosis of many cell surface proteins is triggered following glucose starvation, with recovery from starvation mediated by a small fraction of nutrient transporters preserved at the surface in eisosomes.
影响因子：
3.7
作者：
Borklu Yucel E;Ulgen KO
通讯作者：
Ulgen KO