Preparation of thermo-responsive polymer brushes on hydrophilic polymeric beads by surface-initiated atom transfer radical polymerization for a highly resolutive separation of peptides
Preparation of thermo-responsive polymer brushes on hydrophilic polymeric beads by surface-initiated atom transfer radical polymerization for a highly resolutive separation of peptides
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DOI：
10.1016/j.chroma.2010.07.067
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发表时间：
2010-09-17
影响因子：
4.1
通讯作者：
Okano, Teruo
中科院分区：
文献类型：
--
作者：
Mizutani, Aya;Nagase, Kenichi;Okano, Teruo
采用表面引发原子转移自由基聚合法，在大孔聚甲基丙烯酸缩水甘油酯共聚乙二醇二甲基丙烯酸酯小球表面制备了Poly(N-isopropylacrylamide-co-N-tert-butylacrylamide)[P(IPAAm-co-tBAAm)]刷子，并将其应用于热响应层析固定相。优化的共聚物刷接枝PHMA小球能够分离多肽和蛋白质，峰窄，分辨率高。氮气吸附法测得小球的比表面积为43.0m(2)/g。P(IPAAm-co-tBAAm)接枝PHMA微珠的共聚刷子改进了热响应色谱全水相分离多肽和蛋白质的固定相。(C)2010爱思唯尔B.V.保留所有权利。
Poly(N-isopropylacrylamide-co-N-tert-butylacrylamide) [P(IPAAm-co-tBAAm)] brushes were prepared on poly(hydroxy methacrylate) (PHMA) [hydrolyzed poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate)] beads having large pores by surface-initiated atom transfer radical polymerization (ATRP) and applied to the stationary phases of thermo-responsive chromatography. Optimized amount of copolymer brushes grafted PHMA beads were able to separate peptides and proteins with narrow peaks and a high resolution. The beads were found to have a specific surface area of 43.0 m(2)/g by nitrogen gas adsorption method. Copolymer brush of P(IPAAm-co-tBAAm) grafted PHMA beads improved the stationary phase of thermo-responsive chromatography for the all-aqueous separation of peptides and proteins. (C) 2010 Elsevier B.V. All rights reserved.