β-cell replication and islet neogenesis following partial pancreatectomy
β-cell replication and islet neogenesis following partial pancreatectomy
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DOI：
10.4161/isl.3.4.16338
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发表时间：
2011-07-01
期刊：
影响因子：
2.2
通讯作者：
Levine, Fred
中科院分区：
文献类型：
--
作者：
Lee, Seung-Hee;Hao, Ergeng;Levine, Fred
部分胰腺切除术是研究β细胞再生最常用的模型之一。在这种模型中发生再生的机制一直存在争议，一些人声称胰岛和β细胞新生很重要，而另一些人则声称β细胞复制占主导地位。在这里，我们将时间过程分析与连续BrdU给药相结合，研究部分胰腺切除术后的β细胞再生。虽然再生区的外分泌细胞是高度增殖和阳性的BrdU，再生区的胰岛是阴性的BrdU一周后，部分胰腺切除术，这表明他们是来自预先存在的胰岛，而不是新生的。其他人报告的导管中的胰岛素阳性细胞被视为β细胞新生的证据，这些细胞存在于胰腺的再生区域，但相对不常见且增殖性不高，这表明它们不能解释显著的胰岛新生。与缺乏胰岛新生一致，第二次部分胰腺切除术后的再生区域缺乏胰岛。在部分胰腺切除术后两周可检测到高频率的β-细胞复制，并且在胰腺的再生区域和先前存在的区域中以相似的频率存在。总之，我们的数据表明部分胰腺切除术后不会发生胰岛新生。
Partial pancreatectomy is one of the most commonly used models in the study of beta-cell regeneration. The mechanism by which regeneration occurs in this model has been controversial, with some claiming that islet and beta-cell neogenesis is important, while others claim that beta-cell replication is predominant. Here, we combined a time course analysis with continuous BrdU administration to study beta-cell regeneration following partial pancreatectomy. While exocrine cells in regenerating areas were highly proliferative and positive for BrdU, islets in regenerating areas were negative for BrdU one week after partial pancreatectomy, suggesting that they were derived from preexisting islets rather than being neogenic. The insulin-positive cells in ducts that have been reported by others and taken as evidence of beta-cell neogenesis were present in regenerating regions of the pancreas, but were relatively uncommon and were not highly proliferative, suggesting that they could not account for significant islet neogenesis. Consistent with a lack of islet neogenesis, regenerating areas following a second partial pancreatectomy were devoid of islets. beta-cell replication was detectable at a high frequency two weeks following partial pancreatectomy and was present at a similar frequency in both regenerating and preexisting regions of the pancreas. In summary, our data indicate that islet neogenesis following partial pancreatectomy does not occur.