Dissolution of the maskin-eIF4E complex by cytoplasmic polyadenylation and poly(A)-binding protein controls cyclin B1 mRNA translation and oocyte maturation
Dissolution of the maskin-eIF4E complex by cytoplasmic polyadenylation and poly(A)-binding protein controls cyclin B1 mRNA translation and oocyte maturation
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DOI：
10.1093/emboj/cdf353
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发表时间：
2002-07-15
期刊：
影响因子：
11.4
通讯作者：
Richter, JD
中科院分区：
文献类型：
--
作者：
Cao, QP;Richter, JD
卵母细胞成熟过程中胞质多聚腺苷酸化刺激几种休眠mRNA的翻译。多聚腺苷酸化受细胞质多聚腺苷酸化元件（CPE）及其相关因子CPEB的调节，CPE是反应mRNA 3 '非翻译区的顺式作用元件。CPEB还结合maskin，这种蛋白质反过来又与eIF 4 E（帽结合因子）相互作用。在这里，我们报告说，基于抗体和mRNA报告注射试验，maskin阻止卵母细胞成熟和翻译的CPE-含有细胞周期蛋白B1 mRNA通过阻断协会的eIF 4G与eIF 4 E。maskin-eIF 4 E复合物的解离对于细胞周期蛋白B1 mRNA的翻译激活是必不可少的，并且不仅需要胞质多聚腺苷酸化，还需要poly（A）结合蛋白。这些结果提示了一种分子机制，通过这种机制，含有CPE的mRNA在早期发育中被激活。
Cytoplasmic polyadenylation stimulates the translation of several dormant mRNAs during oocyte maturation in Xenopus. Polyadenylation is regulated by the cytoplasmic polyadenylation element (CPE), a cis-acting element in the 3'-untranslated region of responding mRNAs, and its associated factor CPEB. CPEB also binds maskin, a protein that in turn interacts with eIF4E, the cap-binding factor. Here, we report that based on antibody and mRNA reporter injection assays, maskin prevents oocyte maturation and the translation of the CPE-containing cyclin B1 mRNA by blocking the association of eIF4G with eIF4E. Dissociation of the maskin-eIF4E complex is essential for cyclin B1 mRNA translational activation, and requires not only cytoplasmic polyadenylation, but also the poly(A)-binding protein. These results suggest a molecular mechanism by which CPE- containing mRNA is activated in early development.