Spatial focalization of pheromone/MAPK signaling triggers commitment to cell-cell fusion.
Spatial focalization of pheromone/MAPK signaling triggers commitment to cell-cell fusion.
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DOI：
10.1101/gad.286922.116
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发表时间：
2016-10-01
影响因子：
10.5
通讯作者：
Martin SG
中科院分区：
文献类型：
--
作者：
Dudin O;Merlini L;Martin SG
在这里，Dudin等人。结果表明，细胞融合不需要专门的信号，而是由驱动裂殖酵母早期交配事件的相同信息素-GPCR-MAPK信号级联的空间聚焦触发的。细胞融合是真核生物中普遍存在的受精和发育过程，但这一过程的信号尚不清楚。在这里，我们在裂殖酵母中证明了融合不需要专门的信号，而是由驱动早期交配事件的相同信息素-GPCR(G蛋白偶联受体)-MAPK信号级联的空间聚焦触发的。表达自己信息素受体的自分泌细胞通过将信息素释放集中在融合焦点来触发融合尝试，融合焦点是细胞壁水解酶分泌的动态肌动蛋白。信息素受体和MAPK级联类似地在融合焦点上富含，在野生型交配对中伴随着融合承诺。这种聚焦通过固定融合焦点来促进细胞融合，从而推动局部细胞壁的溶解。我们认为，融合承诺是由融合焦点上MAPK浓度的局部增加强加的，融合焦点的形成与信息素释放和感知的聚焦之间的正反馈推动了融合承诺。
Here, Dudin et al. show that cell fusion does not require a dedicated signal but is triggered by spatial focalization of the same pheromone–GPCR–MAPK signaling cascade that drives earlier mating events in Schizosaccharomyces pombe. Cell fusion is universal in eukaryotes for fertilization and development, but what signals this process is unknown. Here, we show in Schizosaccharomyces pombe that fusion does not require a dedicated signal but is triggered by spatial focalization of the same pheromone–GPCR (G-protein-coupled receptor)–MAPK signaling cascade that drives earlier mating events. Autocrine cells expressing the receptor for their own pheromone trigger fusion attempts independently of cell–cell contact by concentrating pheromone release at the fusion focus, a dynamic actin aster underlying the secretion of cell wall hydrolases. Pheromone receptor and MAPK cascade are similarly enriched at the fusion focus, concomitant with fusion commitment in wild-type mating pairs. This focalization promotes cell fusion by immobilizing the fusion focus, thus driving local cell wall dissolution. We propose that fusion commitment is imposed by a local increase in MAPK concentration at the fusion focus, driven by a positive feedback between fusion focus formation and focalization of pheromone release and perception.
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