Effect of a synthetic link N peptide nanofiber scaffold on the matrix deposition of aggrecan and type II collagen in rabbit notochordal cells
Effect of a synthetic link N peptide nanofiber scaffold on the matrix deposition of aggrecan and type II collagen in rabbit notochordal cells
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DOI：
10.1007/s10856-012-4811-3
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发表时间：
2013-02-01
影响因子：
3.7
通讯作者：
Shao, Zengwu
中科院分区：
文献类型：
--
作者：
Ma, Kaige;Wu, Yongchao;Shao, Zengwu
自组装多肽支架作为三维细胞培养和修复动物组织缺损的生物材料已被广泛研究。然而，很少有研究将肽类支架应用于椎间盘组织工程（IVD）。本研究设计了一种新型的功能化多肽支架，并以脊索细胞（NCs）作为替代细胞来源，研究其生物活性。新型RADA 16-Link N自组装肽支架材料通过直接偶联到生物活性基序Link N而设计。通过混合纯RADA 16-I和RADA 16-Link N（1：1）设计者肽溶液获得连接N肽支架（LN-NS）材料。虽然活/死细胞测定显示LN-NS和RADA 16-I支架材料均与NC具有生物相容性，但与纯RADA 16-I SAP支架材料相比，LN-NS材料显著促进NC粘附。聚集蛋白聚糖和II型胶原的沉积，这是IVD细胞的重要标志物，显着增加。此外，结果表明，连接N基序，髓核的基质类似物，显着促进其他细胞外基质在体外的积累。因此，LN-NS是一种很有前途的生物支架材料，在体外血管组织工程中有着广泛的应用前景。
Self-assembling peptide nanofiber scaffolds have been studied extensively as biological materials for 3-dimensional cell culture and repairing tissue defects in animals. However, few studies have applied peptide nanofiber scaffolds in the tissue engineering of intervertebral discs (IVDs). In this study, a novel functionalized peptide scaffold was specifically designed for IVD tissue engineering, and notochordal cells (NCs) as an alternative cell source for IVD degeneration were selected to investigate the bioactive scaffold material. The novel RADA16-Link N self-assembling peptide scaffold material was designed by direct coupling to a bioactive motif link N. The link N nanofiber scaffold (LN-NS) material was obtained by mixing pure RADA16-I and RADA16-Link N (1:1) designer peptide solutions. Although live/dead cell assays showed that LN-NS and RADA16-I scaffold materials were both biocompatible with NCs, the LN-NS material significantly promoted NC adhesion compared with that of the pure RADA16-I SAP scaffold material. The depositions of aggrecan and type II collagen, which are significant markers for IVD cells, were remarkably increased. Furthermore, the results indicated that the link N motif, the matrix analog of the nucleus pulposus, significantly promoted the accumulation of other extracellular matrices in vitro. We conclude that the novel LN-NS material is a promising biological scaffold material, and may have a broad range of applications in IVD tissue engineering.