Ratiometric fluorescence monitoring of cerebral Cu2 based on coumarin-labeled DNA coupled with the Cu2 -induced oxidation of o-phenylenediamine
Ratiometric fluorescence monitoring of cerebral Cu2 based on coumarin-labeled DNA coupled with the Cu2 -induced oxidation of o-phenylenediamine
复制标题
基于香豆素标记 DNA 与 Cu2 诱导的邻苯二胺氧化的脑 Cu2 比例荧光监测
作者：
Ma Shishi;Zhou Qiao Yu;Mu Fang Ya;Chen Zi Han;Ding Xu Yin;Zhang Min;Shi Guoyue
基于香豆素标记的单链DNA（C-ssDNA）与Cu ~（2+）诱导的邻苯二胺（OPD）氧化反应偶联，建立了一种新的简便的比率荧光法测定Cu ~（2+）。结合微透析技术，比率荧光法也已成功地用于监测脑Cu ~（2+）在大鼠脑组织中的含量，为研究脑Cu ~（2+）相关的生理和病理事件提供了新的机会。
A novel and facile ratiometric fluorescence method for evaluating Cu2+ has been developed based on coumarin-labeled single-stranded DNA (C-ssDNA) coupled with the Cu2+-induced oxidation of o-phenylenediamine (OPD). By combining the microdialysis technique, the ratiometric fluorescence method has also been successfully exploited to monitor the cerebral Cu2+ in the rat brain, promising new opportunities for studying the cerebral Cu2+-associated physiological and pathological events.