Genome-wide analysis of regulation of gene expression and H3K9me2 distribution by JIL-1 kinase mediated histone H3S10 phosphorylation in Drosophila.
Genome-wide analysis of regulation of gene expression and H3K9me2 distribution by JIL-1 kinase mediated histone H3S10 phosphorylation in Drosophila.
复制标题
DOI：
10.1093/nar/gku173
复制
发表时间：
2014-05
影响因子：
14.9
通讯作者：
Johansen KM
中科院分区：
文献类型：
--
作者：
Cai W;Wang C;Li Y;Yao C;Shen L;Liu S;Bao X;Schnable PS;Girton J;Johansen J;Johansen KM
在这项研究中，我们已经确定了全基因组范围内的JIL-1激酶介导的H3 S10磷酸化与基因表达和表观遗传H3 K9 me 2标记的分布的关系。我们在野生型唾液腺细胞中发现，H3 S10 ph标记主要富集在活性基因上，而H3 K9 me 2标记主要与非活性基因相关。从野生型和JIL-1无效突变体幼虫唾液腺中的全球转录谱的比较显示，1539个基因的表达水平变化至少2倍的突变体，这些基因的大量（49%）上调，而51%下调。此外，结果显示，突变体中基因的下调与H3 K9 me 2标记的更高水平或获得相关，而基因的上调与H3 K9二甲基化的丧失或减少相关。这些结果与其中基因表达水平由H3 K9 me 2标记的水平调节的模型相容，所述H3 K9 me 2标记的水平独立于H3 S10 ph标记的状态，这对于转录或基因活化的发生是不需要的。相反，H3 S10磷酸化通过抵消H3 K9二甲基化和基因沉默来间接维持活性转录。
In this study we have determined the genome-wide relationship of JIL-1 kinase mediated H3S10 phosphorylation with gene expression and the distribution of the epigenetic H3K9me2 mark. We show in wild-type salivary gland cells that the H3S10ph mark is predominantly enriched at active genes whereas the H3K9me2 mark is largely associated with inactive genes. Comparison of global transcription profiles in salivary glands from wild-type and JIL-1 null mutant larvae revealed that the expression levels of 1539 genes changed at least 2-fold in the mutant and that a substantial number (49%) of these genes were upregulated whereas 51% were downregulated. Furthermore, the results showed that downregulation of genes in the mutant was correlated with higher levels or acquisition of the H3K9me2 mark whereas upregulation of a gene was correlated with loss of or diminished H3K9 dimethylation. These results are compatible with a model where gene expression levels are modulated by the levels of the H3K9me2 mark independent of the state of the H3S10ph mark, which is not required for either transcription or gene activation to occur. Rather, H3S10 phosphorylation functions to indirectly maintain active transcription by counteracting H3K9 dimethylation and gene silencing.
登录
查看更多内容
影响因子：
7
作者：
Kellner WA;Ramos E;Van Bortle K;Takenaka N;Corces VG
通讯作者：
Corces VG
影响因子：
4.8
作者：
Bao, Xiaomin;Cai, Weili;Johansen, Kristen M.
通讯作者：
Johansen, Kristen M.
DOI：
10.3791/1748
发表时间：
2010-02-09
期刊：
Journal of visualized experiments : JoVE
影响因子：
--
作者：
Cai, Weili;Jin, Ye;Johansen, Kristen M
通讯作者：
Johansen, Kristen M
影响因子：
10.5
作者：
Ebert, A;Schotta, G;Reuter, G
通讯作者：
Reuter, G
DOI：
10.1515/1544-6115.1826
发表时间：
2012-01-01
影响因子：
0.9
作者：
Lund, Steven P.;Nettleton, Dan;Smyth, Gordon K.
通讯作者：
Smyth, Gordon K.