MALDI-TOF mass spectrometry speciation of staphylococci and their discrimination from micrococci isolated from indoor air of schoolrooms
MALDI-TOF mass spectrometry speciation of staphylococci and their discrimination from micrococci isolated from indoor air of schoolrooms
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DOI：
10.1039/b925250a
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发表时间：
2010-01-01
影响因子：
--
通讯作者：
Salzberg, Deborah
中科院分区：
文献类型：
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作者：
Fox, Karen;Fox, Alvin;Salzberg, Deborah
我们工作的重点是从环境中鉴定与医学相关的葡萄球菌;这些生物体是与人类疾病相关的主要机会致病菌。Andersen抽样在学年期间在教室进行。36株分离株（均为革兰氏阳性四分体）中有11株被鉴定为葡萄球菌，23株被鉴定为微球菌。MALDI-TOF MS分析用作分类的第一阶段，随后是标准生化测试，包括API葡萄球菌分析。葡萄球菌分离株均为种属;凝固酶阳性（金黄色葡萄球菌[3株]）和凝固酶阴性：瓦氏葡萄球菌（4株）、人葡萄球菌（2株）、金黄色葡萄球菌（1株）和柯尼葡萄球菌（1株）。S.金黄色葡萄球菌最常见于人的鼻孔中，但经常从皮肤中分离。其他葡萄球菌属是最常从人体皮肤中分离出来的。从室内空气中分离出的微球菌比其他人报道的临床样品中分离出的要多得多。建议在不区分微球菌的情况下，在空气采样中错误鉴定葡萄球菌是常见的。
The focus of our work is the identification of medically relevant staphylococci from the environment; these organisms are among the major opportunistic pathogens associated with human disease. Andersen sampling was performed in schoolrooms during the school year. Eleven of thirty six isolates (all Gram-positive tetrads) were identified as staphylococci and 23 were characterized as micrococci. MALDI-TOF MS profiling was used as the first stage in the classification followed by standard biochemical tests including API Staph profiling. The staphylococcal isolates were each speciated; coagulase positive (Staphylococcus aureus [3 strains]) and coagulase negative: Staphylococcus warneri (4 isolates), Staphylococcus hominis (2), Staphylococcus saprophyticus (1) and Staphylococcus cohnii (1). S. aureus is most commonly found in the human nares but is frequently isolated from skin. The other staphylococcal species are among those most commonly isolated from human skin. Micrococci were much more frequently isolated from indoor air than reported by others for clinical samples. It is suggested that, without discrimination from micrococci, misidentification of staphylococci would be common on air sampling.