Manganese and malonate are individual regulators for the production of lignin and manganese peroxidase isozymes and in the degradation of lignin by Phlebia radiata
Manganese and malonate are individual regulators for the production of lignin and manganese peroxidase isozymes and in the degradation of lignin by Phlebia radiata
复制标题
锰和丙二酸是木质素和锰过氧化物酶同工酶生产以及辐射蛇降解木质素的单独调节剂
DOI：
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发表时间：
1996
影响因子：
5
通讯作者：
A. Hatakka
中科院分区：
文献类型：
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作者：
A. Moilanen;T. Lundell;T. Vares;A. Hatakka
摘要在氮限制培养的白腐菌辐射脉菌中，研究了高锰[180 μM Mn（II）]浓度和添加丙二酸（10 mM）的影响。 单独高水平的锰对木质素过氧化物酶（LiP）、锰过氧化物酶（MNP）或漆酶的产生没有系统的影响。相比之下，含高锰的辐射松培养物在14 C-环标记的合成木质素（[14 C]DHP）的矿化中显示出较低的效率。当不添加丙二酸盐时，低锰（2 μM）培养物的矿化率最高，18天内可达30%。[14 C]DHP的降解基本上受到丙二酸盐的限制。高锰和丙二酸的组合导致MnP和漆酶生产水平的增加，而LiP生产被抑制。此外，MnP和LiP同工酶的表达谱也受到影响。在高锰培养物中发现了一种新的辐射紫菜MnP同工酶，其等电点为3.6（MnP 3）。此外，丙二酸单独引起一些镇压，但也刺激独特的MNP和LiP同工酶的影响。结果表明，锰和丙二酸是辐射紫菜木质素降解过程中MNP和LiP表达的独立调控因子，且在辐射紫菜木质素降解过程中的作用不同。
Abstract The effects of high manganese [180 μM Mn(II)] concentration and addition of malonate (10 mM) were studied in nitrogen-limited cultures of the white-rot fungus, Phlebia radiata. High levels of manganese alone showed no systematic influence on the production of lignin peroxidase (LiP), manganese peroxidase (MnP) or laccase. In contrast, high-manganese containing cultures of P. radiata showed lower efficiency in the mineralization of 14C-ring-labelled synthetic lignin ([14C]DHP). The highest rates of mineralization, up to 30% in 18 days, were reached in low- manganese(2 μM)-containing cultures when malonate was omitted. Degradation of [14C]DHP was substantially restricted by the addition of malonate. The combination of high manganese and malonate resulted in increased levels of MnP and laccase production, whereas LiP production was repressed. Also, the profiles of expression of the MnP and LiP isozymes were affected. A new P. radiata MnP isozyme of pI 3.6 (MnP3) was found in the high-manganese cultures. Addition of malonate alone caused some repression but also stimulating effects on distinctive MnP and LiP isozymes. The results indicate that manganese and malonate are individual regulators of MnP and LiP expression and have different roles in the degradation of lignin by P. radiata.