DEVELOPMENT OF THE ENDOPIRIFORM NUCLEUS AND THE CLAUSTRUM IN THE RAT-BRAIN
DEVELOPMENT OF THE ENDOPIRIFORM NUCLEUS AND THE CLAUSTRUM IN THE RAT-BRAIN
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DOI：
10.1016/0306-4522(91)90236-h
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发表时间：
1991-01-01
期刊：
影响因子：
3.3
通讯作者：
ALTMAN, J
中科院分区：
文献类型：
--
作者：
BAYER, SA;ALTMAN, J
用[H-3]胸腺嘧啶核苷放射自显影法定量测定了出生后第60天处死的大鼠，其母鼠在胚胎日龄E13和E14，E14和E15连续两天每天注射[H-3]胸腺嘧啶核苷后，其内虹膜状核和屏状核神经元的起源时间。E21和E22。屏状体起源较晚，在E15和E16，并且在纵向方向上具有很强的梯度，从后（较老）到前（较年轻）。相反，内虹膜状核起源早，在E14和E15，缺乏纵向梯度，但在垂直方向，腹侧（老年人）背侧（年轻人）有一个强大的。本文用顺序存活[H-3]胸腺嘧啶核苷放射自显影术定性地确定了胚胎大鼠内虹膜状神经元和锁状神经元的来源和定居点。母鼠在E14（重度标记较老的内虹膜状神经元）或E16（重度标记较年轻的锁状神经元）接受[H-3]胸苷单次注射，并在连续24 h间隔内处死。推测的梨状核内的神经元在其神经发生高峰后2 ~ 3天内稳定下来，而推测的屏状核内的神经元在其神经发生高峰后约5天才稳定下来。推测梨状核内的神经元产生于苍白球纹状体室角，即形成新皮层原基和基底神经节之间的楔形的神经上皮，并且在新皮层神经上皮中产生了幽闭神经元。不同的发展模式之间的虹膜状核和屏状核支持的解剖学证据，这些核团有不同的连接。此外，屏状核中的神经发生梯度与屏状核和新皮质之间的解剖学连接模式相关。
Long-survival [H-3]thymidine autoradiography was used to quantitatively determine the time of origin of neurons in the endopiriform nucleus and the claustrum in rats killed on postnatal day 60 after their dams received two consecutive daily injections of [H-3]thymidine on embryonic day E13 and E14, E14 and E15.... E21 and E22. The claustrum originates late, on E15 and E16, and has a strong gradient in the longitudinal direction, posterior (older) to anterior (younger). In contrast, the endopiriform nucleus originates early, on E14 and E15, and lacks a longitudinal gradient but has a strong one in the vertical direction, ventral (older) to dorsal (younger). Sequential-survival [H-3]thymidine autoradiography was used to qualitatively determine the germinal sources and settling sites of endopiriform and claustral neurons in embryonic rats. The dams received a single injection of [H-3]thymidine on either E14 (to heavily label older endopiriform neurons) or E16 (to heavily label younger claustral neurons) and were killed in sequential 24-h intervals. Neurons in the presumptive endopiriform nucleus settle within two to three days after their peak time of neurogenesis while those in the presumptive claustrum take approximately five days to settle after their peak.It is postulated that endopiriform neurons are generated in the palliostriatal ventricular angle, the neuroepithelium that forms a wedge between the primordia of the neocortex and the basal ganglia, and that claustral neurons are generated in the neocortical neuroepithelium. Divergent developmental patterns between the endopiriform nucleus and the claustrum support the anatomical evidence that these nuclei have different connections. Furthermore, neurogenetic gradients in the claustrum correlate with the pattern of anatomical connections between the claustrum and the neocortex.