ON THE COMPLEXITY OF SELF
ON THE COMPLEXITY OF SELF
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DOI：
10.1038/353660a0
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发表时间：
1991-10-17
期刊：
影响因子：
64.8
通讯作者：
JANEWAY, CA
中科院分区：
文献类型：
--
作者：
RUDENSKY, AY;RATH, S;JANEWAY, CA
与自身主要组织相容性复合体（MHC）分子结合的SELF肽在胸腺内发育期间参与T细胞的阳性1，2和阴性3，4选择。我们在这里报告了新型MHC限制性单克隆抗体Y-Ae（参考文献5）检测主要自身肽6的MHC II类结合形式。Y-Ae结合自身抗原呈递细胞上约12%的相关MHC II类分子。由Y-Ae检测的肽是从MHC分子6洗脱的几种主要肽之一。这些数据表明，由自身MHC II类分子以足以向CD 4 T细胞发出信号的密度呈递的自身肽具有非常有限的复杂性。此外，由于Y-Ae染色介导阴性选择的抗原呈递细胞，但不染色驱动阳性选择的胸腺皮质上皮细胞5，因此自身肽：自身MHC II类复合物的差异表达可能是胸腺内选择的关键特征。
SELF peptides bound to self major histocompatibility complex (MHC) molecules have been implicated both in positive 1,2 and in negative 3,4 selection of T cells during intrathymic development. We report here that the novel MHC-restricted monoclonal antibody Y-Ae (ref. 5) detects the MHC class II bound form of a major self peptide 6. Y-Ae binds approximately 12% of the relevant MHC class II molecules on self antigen presenting cells. The peptide detected by Y-Ae is one of several major peptides eluted from the MHC molecule 6. These data suggest that self peptides presented by self MHC class II molecules at densities sufficient to signal a CD4 T cell are of very limited complexity. Furthermore, as Y-Ae stains antigen presenting cells that mediate negative selection but not thymic cortical epithelial cells 5 that drive positive selection 3, differential expression of self peptide:self MHC class II complexes may be a key feature of intrathymic selection.