Patterns of [3H] thymidine incorporation differ in immature rats and mature, cycling rats.
Patterns of [3H] thymidine incorporation differ in immature rats and mature, cycling rats.
复制标题
[3H]胸苷掺入模式在未成熟大鼠和成熟、循环大鼠中不同。
DOI：
10.1095/biolreprod34.1.229
复制
发表时间：
1986
影响因子：
3.6
通讯作者：
Hirshfield,AN
中科院分区：
文献类型：
--
作者：
Hirshfield,AN
当卵泡发育到排卵前阶段时，毗邻基底膜的颗粒细胞不再含有[~3H]胸腺嘧啶核苷(~H-TdR)。本实验的目的是确定在卵泡生长过程中，这些壁层颗粒细胞的细胞增殖何时停止。放射自显影是在体内连续输注或在体外与~3H-TdR孵育后拍摄的。在周期大鼠，颗粒层壁层上的银粒浓度低于大多数卵泡的窦部，最大横截面积(LCS)中有1000个细胞。这种向心性标记模式随着卵泡大小的增加而变得更加显著。发情前期，只有前韧带铁饼细胞(卵丘和卵丘卵母细胞复合体的卵泡壁支撑部分)继续掺入~3H-TdR。与骑自行车的大鼠相比，青春期前大鼠的卵巢中没有向心标记模式，即使在相同大小的卵泡中也是如此。这两种动物之间卵泡生长模式的差异表明，周期促性腺激素激增对颗粒细胞增殖的控制有影响。
By the time follicular development has progressed to the preovulatory stage, granulosa cells abutting the basement membrane no longer incorporate [3H] thymidine (3H-TdR). The purpose of this experiment was to determine when, during the course of follicular growth, cell proliferation in these mural granulosa cells ceases. Autoradiographs were prepared following continuous 3H-TdR infusion in vivo, or incubation with 3H-TdR in vitro. In cycling rats, the concentration of silver grains over mural regions of the granulosa layer was lower than over antral regions of most follicles with >1000 cells in the largest cross section (LCS). This centripetal labeling pattern became more striking as follicular size increased. By proestrus, only the cells of the discus proligerus (cumulus and the portion of the follicular wall supporting the cumulus oocyte complex) continued to incorporate 3H-TdR. In contrast to cycling rats, centripetal labeling patterns were not seen in ovaries of prepubertal rats, even in follicles of the same size. The difference in follicular growth patterns between these two types of animals suggests an influence of cyclic gonadotropin surges on the control of granulosa cell proliferation.