Complete genome ancalysis of 33 ecologically and biologically diverse rift valley fever virus strains reveals widespread virus movement and low genetic diversity due to recent common ancestry
Complete genome ancalysis of 33 ecologically and biologically diverse rift valley fever virus strains reveals widespread virus movement and low genetic diversity due to recent common ancestry
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DOI：
10.1128/jvi.02095-06
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发表时间：
2007-03-01
影响因子：
5.4
通讯作者：
Nichol, Stuart T.
中科院分区：
文献类型：
--
作者：
Bird, Brian H.;Khristova, Marina L.;Nichol, Stuart T.
裂谷热(RVF)病毒是一种由蚊子传播的RNA病毒，导致非洲人和牲畜急性发热病的大规模爆炸性暴发，造成重大死亡和经济影响。从1944年至2000年，从整个非洲和沙特阿拉伯收集的33株不同的裂谷热病毒株，包括在疾病模型中致病性不同的株，成功地高通量地生成了完整的基因组序列。虽然已经确定了几种不同的病毒遗传谱系，这与地理起源大致相关，但也发现了指示病毒远距离移动的多个例外。在疫情中分离的病毒株(例如毛里塔尼亚、1987年或埃及，1977至1978年)表现出很少的多样性，而在流行病环境中(例如，1970年代的津巴布韦)分离的病毒株可能高度多样化。此外，2000年沙特阿拉伯裂谷热的大规模暴发似乎涉及从东非传入病毒，这是基于1998年东非病毒的近亲关系。病毒的遗传多样性很低(接近5%)，主要涉及突变的积累，平均2.9x10(-4)个S/位点/年，尽管发现了一些RNA片段重组的证据。对当前裂谷热病毒遗传多样性的贝叶斯分析将这些病毒最近的共同祖先放在19世纪末，也就是非洲的殖民时期，当时农业实践发生了戏剧性的变化，引入了非本土牲畜品种。除了对裂谷热病毒的进化和生态的洞察外，这些基因组数据还为设计分子检测分析和原型疫苗提供了基础，有助于对抗这种重要的疾病。
Rift Valley fever (RVF) virus jis a mosquito-borne RNA virus responsible for large explosive outbreaks of acute febrile disease in humans and livestock in Africa with significant mortality and economic impact. The successful high-throughput generation of the complete genome sequence was achieved for 33 diverse RVF virus strains collected from throughout Africa and Saudi Arabia from 1944 to 2000, including strains differing in pathogenicity in disease models. While several distinct virus genetic lineages were determined, which approximately correlate with geographic origin, multiple exceptions indicative of long-distance virus movement have been found. Virus strains isolated within an epidemic (e.g., Mauritania, 1987, or Egypt, 1977 to 1978) exhibit little diversity, while those in enzootic settings (e.g., 1970s Zimbabwe) can be highly diverse. In addition, the large Saudi Arabian RVF outbreak in 2000 appears to have involved virus introduction from East Africa, based on the close ancestral relationship of a 1998 East African virus. Virus genetic diversity was low (similar to 5%) and primarily involved accumulation of mutations at an average of 2.9 x 10(-4) substitution s/site/year, although some evidence of RNA segment reassortment was found. Bayesian analysis of current RVF virus genetic diversity places the most recent common ancestor of these viruses in the late 1800s, the colonial period in Africa, a time of dramatic changes in agricultural practices and introduction of nonindigenous livestock breeds. In addition to insights into the evolution and ecology of RVF virus, these genomic data also provide a foundation for the design of molecular detection assays and prototype vaccines useful in combating this important disease.