Carotenoid distribution in living cells of Haematococcus pluvialis (Chlorophyceae).
Carotenoid distribution in living cells of Haematococcus pluvialis (Chlorophyceae).
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DOI：
10.1371/journal.pone.0024302
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Timlin JA
中科院分区：
文献类型：
--
作者：
Collins AM;Jones HD;Han D;Hu Q;Beechem TE;Timlin JA
雨生红球藻是一种淡水单细胞绿色微藻，属于绿藻纲，因其能够积累大量红色酮类胡萝卜素虾青素（3,3'-二羟基-β,β-胡萝卜素-4,4'-二酮）而具有商业价值。使用共焦拉曼显微镜和多变量分析，我们展示了光谱解析与虾青素和β-胡萝卜素以及叶绿素荧光相关的共振增强拉曼特征的能力。通过在数学上分离这些光谱特征，反过来，可以在生命周期的各个阶段的雨生水球菌活细胞中相互独立地定位这些物种。仅在叶绿体中发现叶绿素发射，而在细胞质空间的球状和点状区域内发现了虾青素。此外，我们发现了β-胡萝卜素与细胞质中的叶绿体和虾青素共存的证据。这些观察结果表明β-胡萝卜素是虾青素的前体，并且虾青素的合成发生在叶绿体之外。我们的工作证明了共焦拉曼显微镜在解析活细胞中高度相似的发色团的光谱特征方面的广泛用途。
Haematococcus pluvialis is a freshwater unicellular green microalga belonging to the class Chlorophyceae and is of commercial interest for its ability to accumulate massive amounts of the red ketocarotenoid astaxanthin (3,3′-dihydroxy-β,β-carotene-4,4′-dione). Using confocal Raman microscopy and multivariate analysis, we demonstrate the ability to spectrally resolve resonance–enhanced Raman signatures associated with astaxanthin and β-carotene along with chlorophyll fluorescence. By mathematically isolating these spectral signatures, in turn, it is possible to locate these species independent of each other in living cells of H. pluvialis in various stages of the life cycle. Chlorophyll emission was found only in the chloroplast whereas astaxanthin was identified within globular and punctate regions of the cytoplasmic space. Moreover, we found evidence for β-carotene to be co-located with both the chloroplast and astaxanthin in the cytosol. These observations imply that β-carotene is a precursor for astaxanthin and the synthesis of astaxanthin occurs outside the chloroplast. Our work demonstrates the broad utility of confocal Raman microscopy to resolve spectral signatures of highly similar chromophores in living cells.
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影响因子：
2.1
作者：
Heraud, Philip;Beardall, John;Wood, Bayden R.
通讯作者：
Wood, Bayden R.
影响因子：
2.4
作者：
Jones, Howland D. T.;Haaland, David M.;Pedroso, M. Cristina
通讯作者：
Pedroso, M. Cristina
影响因子：
3.3
作者：
BOROWITZKA, MA;HUISMAN, JM;OSBORN, A
通讯作者：
OSBORN, A
影响因子：
3.3
作者：
Grünewald, K;Hagen, C
通讯作者：
Hagen, C
影响因子：
2.9
作者：
ALLEN, MM
通讯作者：
ALLEN, MM