Expression of heat shock protein 60 in the tissues of transported piglets
Expression of heat shock protein 60 in the tissues of transported piglets
复制标题
热休克蛋白60在转运仔猪组织中的表达
DOI：
10.1007/s12192-008-0055-z
复制
发表时间：
2009-01-01
影响因子：
3.8
通讯作者：
Hartung, Joerg
中科院分区：
文献类型：
--
作者：
Zhu, Lin;Bao, Endong;Hartung, Joerg
在本研究中，我们试图确定热休克蛋白60 （Hsp60）在运输仔猪组织中的分布和表达。试验选取24头平均体重为20 +/- 1 kg的中国二华联仔猪，分别在2 h运输和正常饲养条件下进行试验。酶学分析结果显示，2 h后仔猪血清肌酸激酶和天冬氨酸转氨酶浓度显著升高。转运2小时后，经组织病理学检查证实，心脏、肝脏和肾脏的实质细胞出现颗粒状和空泡变性的急性细胞病变。这些结果表明，运输应激可引起心脏、肝脏和肾脏的组织损伤。心肌细胞、肝细胞、肾小管上皮细胞、基底腺上皮细胞细胞质中均可见hsp60阳性免疫染色。然而，酶联免疫吸附试验结果显示，Hsp60仅在胃中表达显著升高，在心脏中表达较低，肝脏和肾脏中Hsp60表达升高趋势不显著。这些结果表明，不同组织对运输应激的敏感性不同，可能导致Hsp60在不同组织中的细胞保护水平不同。运输2 h后，Hsp60的表达与心脏和胃细胞保护功能的减弱相一致。然而，Hsp60在心脏和胃组织细胞保护中的直接作用还有待进一步研究。
In this study, we sought to determine the distribution and expression of heat shock protein 60 (Hsp60) in the tissues of transported piglets. A total of 24 Chinese Erhualian piglets with an average body weight of 20 +/- 1 kg were assessed under both 2-h transported and normal housing conditions. Results of enzymatic analysis showed that the serum creatine kinase and aspartate aminotransferase concentrations were significantly increased in the 2-h transported piglets. Acute cellular lesions characterized by granular and vacuolar degeneration of the parenchyma cells in the tested heart, liver, and kidney were also confirmed by histopathological test after 2 h transportation. These results indicate that transport stress induces tissue damage to heart, liver, and kidney. Hsp60-positive immunostaining was consistently detected in the cytoplasm of myocardial cells, hepatocytes, renal tubular epithelial cells, and epithelial cells of fundic gland. However, results of enzyme-linked immunosorbent assay indicated that Hsp60 expression was only significantly elevated in the stomach, with lower expression in the heart and a non-significant trend of increased liver and kidney expression of Hsp60. These results indicate that different tissues had different sensitivities to transport stress, possibly resulting in varying levels of cytoprotection by Hsp60 in the different tissues. The expression of Hsp60 following 2 h transportation coincided with deterioration of cardiac cytoprotection in the heart and protection in the stomach. However, the direct role of Hsp60 in cytoprotection of heart and stomach tissues needs further investigation.