Microencapsulation of Bifidobacterium bifidum F‐35 in reinforced alginate microspheres prepared by emulsification/internal gelation
Microencapsulation of Bifidobacterium bifidum F‐35 in reinforced alginate microspheres prepared by emulsification/internal gelation
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DOI：
10.1111/j.1365-2621.2011.02685.x
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发表时间：
2011-08
影响因子：
3.3
通讯作者：
Qi-chang Zou;Jianxin Zhao;Xiaoming Liu;F. Tian;He-ping Zhang;Hao Zhang;Wei Chen
中科院分区：
文献类型：
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作者：
Qi-chang Zou;Jianxin Zhao;Xiaoming Liu;F. Tian;He-ping Zhang;Hao Zhang;Wei Chen
通过乳化/内部凝胶化制备的含有两歧双歧杆菌F-35的藻酸盐微球通过与果胶或淀粉共混或用壳聚糖或聚-L-赖氨酸包衣来增强，以提供对菌株的额外保护。研究了这些处理方法对微球粒径、包封率的影响以及微囊化对细胞的保护作用。在不同类型的加固中没有检测到EY的差异，约为43- 50%。增强海藻酸钠微球的平均粒径在117 ~ 178 μm之间，当淀粉加入海藻酸钠基质中时达到最大值。据观察，保护效果与类型的钢筋。而壳聚糖包被的海藻酸钠微球在模拟胃肠道环境和4 °C保存1个月内对微囊化细胞的保护效果最好，可作为双歧杆菌肠道递送的较有效载体。
Summary Alginate microspheres containing Bifidobacterium bifidum F-35 prepared by emulsification/internal gelation were reinforced by blending with pectin or starch or coating with chitosan or poly-L-lysine to provide extra protection for the strain. The influence of these treatments on the size of microspheres, encapsulation yield (EY) and protective effect of microencapsulation on the cells was studied. No difference was detected in EY with different types of reinforcement, which was approximately 43–50%. The mean diameter of reinforced alginate microspheres ranged from 117 to 178 μm, reaching a maximum value when starch was incorporated in the alginate matrix. It was observed that the protective effects varied with the type of reinforcement. However, chitosan-coated alginate microspheres provided the best protection for microencapsulated cells in simulated gastrointestinal tract and during 1 month of storage at 4 °C, and this system could be the comparatively effective vector of bifidobacteria for intestinal delivery.