Characterization of variability and genetic similarity of European pear using microsatellite loci developed in apple
Characterization of variability and genetic similarity of European pear using microsatellite loci developed in apple
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DOI：
10.1016/j.scienta.2007.02.002
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发表时间：
2007-06
影响因子：
4.3
通讯作者：
A. Wünsch;J. Hormaza
中科院分区：
文献类型：
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作者：
A. Wünsch;J. Hormaza
利用在苹果中开发的7个SSR位点，对63个欧洲梨品种进行了鉴定。共扩增46个片段，平均每个SSR扩增6.6个等位基因。只有一个微卫星扩增了一个以上的位点。6个单位点ssr的平均期望杂合度和观察杂合度分别为0.68和0.44，每个位点的有效等位基因数为3.43。扩增的片段产生了61种不同的指纹模式，可以明确区分所有被分析的品种。只有两个由突变产生的品种不能与原品种区分开来。对估计的遗传相似性进行聚类分析，根据品种的家系和地理来源进行分类。与在蔷薇科其他水果作物中检测到的变异性相比，在欧洲梨品种中检测到的SSRs变异性较低。
Seven microsatellite loci (SSR) developed in apple were used for the identification of 63 European pear cultivars. A total of 46 fragments were amplified, with an average of 6.6 alleles per SSR. Only one microsatellite amplified more than one locus. The mean expected and observed heterozygosities over the six single-locus SSRs averaged 0.68 and 0.44, respectively, and the number of effective alleles per loci was 3.43. The amplified fragments produced 61 different fingerprinting patterns that allowed to unequivocally distinguish all the varieties analyzed. Only two varieties, which derive from mutations, could not be distinguished from the original variety. Cluster analysis of the estimated genetic similarity, grouped the varieties according to their pedigree and their geographic origin. The variability detected with the SSRs in European pear varieties was low when compared with the variability detected in other fruit crops in the Rosaceae.