Accumulation and phytotoxicity of perfluorooctanoic acid and 2,3,3,3-tetrafluoro-2-(heptafluoropropoxy)propanoate in Arabidopsis thaliana and Nicotiana benthamiana
Accumulation and phytotoxicity of perfluorooctanoic acid and 2,3,3,3-tetrafluoro-2-(heptafluoropropoxy)propanoate in Arabidopsis thaliana and Nicotiana benthamiana
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DOI：
10.1016/j.envpol.2019.113817
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发表时间：
2020-04-01
影响因子：
8.9
通讯作者：
Chu, Kung-Hui
中科院分区：
文献类型：
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作者：
Chen, Chih-Hung;Yang, Shih-Hung;Chu, Kung-Hui
2，3，3，3-Tetrafluoro-2-(heptafluoropropoxy)propanoate(称为GenX)已被用作全氟辛酸的替代品，全氟辛酸已于2015年逐步淘汰用于工业和消费品的配方。虽然已经研究了GenX对实验室动物的影响，但对其对植物的影响知之甚少。本研究检测和比较了GenX和PFOA在模式植物拟南芥和烟草体内的积累和毒性。两种植物在暴露于全氟辛酸或GenX后都表现出生物量和根生长的减少，并呈剂量依赖关系。GenX和全氟辛酸的生物积累因子(BF)随植物种类的不同而不同，海棠的BF高于北冰草。此外，GenX和全氟辛酸在地上部组织中的积累量比北冰洋更容易积累。暴露于GenX还导致叶绿素含量(18%)和总酚类化合物(26%)下降。然而，GenX暴露增加了本氏沼虾超氧化物歧化酶活性和过氧化氢含量(分别增加1.6倍和2.6倍)。总体而言，我们的结果表明GenX是生物积累的，它的积累可能会抑制植物的生长和光合作用，并诱导氧化胁迫。(C)2019爱思唯尔有限公司。保留所有权利。
2,3,3,3-Tetrafluoro-2-(heptafluoropropoxy)propanoate (known as GenX) has been used as an alternative to perfluorooctanoic acid (PFOA) which was phased out of formulations for industrial and consumer product applications in 2015. While the effects of GenX on lab animals have been studied, little is known about its effects on plants. This study examined and compared the accumulation and toxicity of GenX and PFOA in the model plants Arabidopsis thaliana and Nicotiana benthamiana. Both plants showed reduction in biomass and root growth following exposure to PFOA or GenX in a dosage-dependent manner. The bioaccumulation factors (BFs) of GenX and PFOA were plant species-dependent, with higher BFs in A. thaliana compared to N. bethanminana. Additionally, GenX and PFOA were more readily accumulated into shoot tissues of A. thaliana than in N. bethanminana. Exposure to GenX also caused a reduction in chlorophyll content (18%) and total phenolic compounds (26%). However, GenX exposure increased superoxide dismutase activity and H2O2 content (1.6 and 2.6 folds increase, respectively) in N. benthamiana. Overall, our result suggest that GenX is bioaccumulative, and that its accumulation likely inhibits plant growth and photosynthesis as well as inducing oxidative stress. (C) 2019 Elsevier Ltd. All rights reserved.