Similarities in murine infection and immune response to Borrelia bissettii and Borrelia burgdorferi sensu stricto.
Similarities in murine infection and immune response to Borrelia bissettii and Borrelia burgdorferi sensu stricto.
复制标题
鼠类感染和对伯氏疏螺旋体和严格伯氏疏螺旋体的免疫反应的相似性。
DOI：
10.1099/mic.0.000192
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Liang,FangTing
中科院分区：
文献类型：
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作者：
LeydetJr,BrianF;Liang,FangTing
1982年，伯氏疏螺旋体被鉴定为莱姆病的病原体。从那时起，越来越多的伯氏疏螺旋体在美国被分离出来。到目前为止，尽管越来越多的证据表明这些物种与人类疾病有关，但其中许多物种的研究仍然不足。比塞氏疏螺旋体是一种与Tob密切相关的螺旋体。与人类疾病有松散联系的伯多菲病毒。使用实验性小鼠感染模型，我们比较了传染性和体液免疫反应与北美分离株OFB。比塞蒂和B。伯格氏菌培养、分子和血清学方法。原来的B。菊花培养物不能感染免疫能力强的小鼠，但在免疫缺陷动物适应后被证实是有感染性的。在皮肤、心脏和膀胱中，可以观察到明显较轻的关节组织的负荷。用免疫印迹法和C6-EL ISA法检测伯氏杆菌。此外，该分离物OFB。在Ion Torrent PGM上测序，成功地鉴定了许多与INB描述的哺乳动物毒力因子同源的基因。勃格多费里。在这个特征良好的小鼠模型中，两种感染之间的相似之处有助于我们理解B的潜在致病性质。比塞蒂。芽孢杆菌的感染动态。Bissettii，尤其是诱导的体液反应，与Tob相似。Burgdorferi，提示该种可能对人类疏螺旋体病的流行病学有贡献。
In 1982,Borrelia burgdorferi sensu stricto(ss) was identified as the aetiological agent of Lyme disease. Since then an increasing number ofBorrelia burgdorferi sensu lato(sl) species have been isolated in the United States. To date, many of these species remain understudied despite mounting evidence associating them with human illness.Borrelia bissettiiis a spirochaete closely related toB. burgdorferithat has been loosely associated with human illness. Using an experimental murine infection model, we compared the infectivity and humoral immune response with a North American isolate ofB. bissettiiandB. burgdorferiusing culture, molecular and serological methods. The originalB. bissettiicultures were unable to infect immunocompetent mice, but were confirmed to be infectious after adaptation in immunodeficient animals.B. bissettiiinfection resulted in spirochaete burdens similar toB. burgdorferiin skin, heart and bladder whereas significantly lower burdens were observed in the joint tissues.B. bissettiiinduced an antibody response similar toB. burgdorferias measured by both immunoblotting and the C6 ELISA. Additionally, this isolate ofB. bissettiiwas sequenced on the Ion Torrent PGM, which successfully identified many genes orthologous to mammalian virulence factors described inB. burgdorferi. Similarities seen between both infections in this well-characterized murine model contribute to our understanding of the potential pathogenic nature ofB. bissettii. Infection dynamics ofB. bissettii,and especially the induced humoral response, are similar toB. burgdorferi, suggesting this species may contribute to the epidemiology of human borreliosis.