Phase contrast microscopy with fringe contrast adjustable by using grating-based phase-shifter.

Phase contrast microscopy with fringe contrast adjustable by using grating-based phase-shifter.
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DOI:
10.1364/oe.20.016077
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发表时间:
2012-07
期刊:
影响因子:
3.8
通讯作者:
Juanjuan Zheng;Baoli Yao;Peng Gao;Tong Ye
Juanjuan Zheng;Baoli Yao;Peng Gao;Tong Ye
中科院分区:
物理与天体物理2区
文献类型:
--
作者:
Juanjuan Zheng;Baoli Yao;Peng Gao;Tong Ye

文献摘要

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本文提出了一种条纹对比度可调的新相衬方法。在物波的傅里叶平面中,两个朗奇光栅,即中心光栅和周围光栅,分别用于调制非衍射和衍射分量的相位。通过将两个光栅分别加载在空间光调制器上,可以独立测量非衍射和衍射分量,从而大大简化了重建过程。此外,可以通过改变两个光栅的调制深度来调节相衬干涉图的条纹对比度。通过理论分析和实验验证了该方法的可行性。
In this paper a new phase contrast method with fringe contrast adjustable is proposed. In the Fourier plane of the object wave, two Ronchi gratings i.e., a central grating and a surrounding grating, are used to modulate the phases of the undiffracted and diffracted components, respectively. By loading the two gratings separately on spatial light modulator, the undiffracted and diffracted components can be measured independently, which simplify greatly the reconstruction process. Besides, the fringe contrast of the phase contrast interferogram can be adjusted by changing the modulation depth of the two gratings. The feasibility of the proposed method is verified by theoretical analysis and experiment.