Engineering Arsenic Tolerance and Hyperaccumulation in Plants for Phytoremediation by a PvACR3 Transgenic Approach
Engineering Arsenic Tolerance and Hyperaccumulation in Plants for Phytoremediation by a PvACR3 Transgenic Approach
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通过 PvACR3 转基因方法进行植物修复的砷耐受性和超积累
DOI：
10.1021/es4012096
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发表时间：
2013-08-20
影响因子：
11.4
通讯作者：
Ma, Mi
中科院分区：
文献类型：
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作者：
Chen, Yanshan;Xu, Wenzhong;Ma, Mi
砷(As)污染是一个全球性的问题，因此，以植物为基础的污染土壤的清理，即植物修复，引起了人们的极大兴趣。最近，转基因方法被设计成植物修复技术。在此，我们利用单基因转基因方法研究了拟南芥对砷的耐受性和积累。PvACR3是超积累蕨类植物Pteris vittata中的一个关键的亚砷酸盐[As(III)]逆向转运蛋白，在CaMV 35S启动子的驱动下在拟南芥中表达。在AS处理下，转PvACR3基因植株的耐性显著提高。PvACR3转基因种子甚至可以在对野生型种子致死的80亩砷(III)或1200亩砷[As(V)]处理下萌发和生长。在短期实验中，PvACR3定位于拟南芥的质膜上，并增加亚砷酸盐向外部介质的外流。砷测定表明，PvACR3显著降低了根中As的浓度，同时增加了地上部As(V)的含量。当种植在含As(V)的土壤中(10ppm As)时，转基因植物在地上组织中积累的As大约是野生型植物的7.5倍。这项研究为了解PvACR3的行为和As在植物中的代谢生理提供了重要的见解。我们的工作还提供了一种简单实用的PvACR3转基因方法，用于工程设计耐砷和超积累植物进行植物修复。
Arsenic (As) pollution is a global problem, and the plant-based cleanup of contaminated soils, called phytoremediation, is therefore of great interest. Recently, transgenic approaches have been designed to develop As phytoremediation technologies. Here, we used a one-gene transgenic approach for As tolerance and accumulation in Arabidopsis thaliana. PvACR3, a key arsenite [As(III)] antiporter in the As hyperaccumulator fern Pteris vittata, was expressed in Arabidopsis, driven by the CaMV 35S promoter. In response to As treatment, PvACR3 transgenic plants showed greatly enhanced tolerance. PvACR3 transgenic seeds could even germinate and grow in the presence of 80 mu M As(III) or 1200 mu M arsenate [As(V)] treatments that were lethal to wildtype seeds. PvACR3 localizes to the plasma membrane in Arabidopsis and increases arsenite efflux into external medium in short-term experiments. Arsenic determination showed that PvACR3 substantially reduced As concentrations in roots and simultaneously increased shoot As under 150 mu M As(V). When cultivated in As(V)-containing soil (10 ppm As), transgenic plants accumulated approximately 7.5-fold more As in above-ground tissues than wild-type plants. This study provides important insights into the behavior of PvACR3 and the physiology of As metabolism in plants. Our work also provides a simple and practical PvACR3 transgenic approach for engineering As-tolerant and -hyperaccumulating plants for phytoremediation.