Inoculation of new rhizobial isolates improve nutrient uptake and growth of bean (Phaseolus vulgaris) and arugula (Eruca sativa).
Inoculation of new rhizobial isolates improve nutrient uptake and growth of bean (Phaseolus vulgaris) and arugula (Eruca sativa).
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DOI：
10.1002/jsfa.7527
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发表时间：
2016-08
影响因子：
4.1
通讯作者：
Eduardo M. de Souza;V. L. Bassani;R. A. Sperotto;C. Granada
中科院分区：
文献类型：
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作者：
Eduardo M. de Souza;V. L. Bassani;R. A. Sperotto;C. Granada
背景技术在当前的农业模式中，化肥的大量使用造成了环境和经济损失。接种植物促生长（PGP）固氮细菌是使用氮肥、磷肥和钾肥的替代方法。在这项研究中，对从菜豆（Phaseolus vulgaris）根瘤中分离出的根瘤菌菌株进行了评估，以期鉴定出一种能够改善菜豆发芽和生长的高效固氮根瘤菌菌株。结果从巴西南部7个地点采集了菜豆植物，获得了210株本土根瘤菌分离株。 PGP性状评价表明，大多数根瘤菌分离株是非铁载体生产者和弱吲哚类化合物生产者。在实验室条件下，根瘤菌分离株 E15（豆科根瘤菌）和 L5（放射根瘤菌）可提高菜豆和芝麻菜 (Eruca sativa) 幼苗的发芽率、长度和干重。在温室条件下，接种根瘤菌分离株 VC28 和 L15（均为豆根瘤菌）的普通豆类植物呈现出最高的根瘤数和地上部干物质，而 VC28 也呈现出最高的地上部氮和钾值。分离株 L17 表现出高效的固氮作用，即使结瘤减少。结论 这些新的根瘤菌分离株是有吸引力的 PGP 化肥替代品。 © 2015 中国化学工业学会。
BACKGROUND In the current agricultural model, the massive use of chemical fertilizer causes environmental and economic losses. Inoculation of plant-growth-promoting (PGP) nitrogen-fixing bacteria is an alternative to the use of nitrogen, phosphorus and potassium fertilizers. In this study, rhizobia strains isolated from common bean (Phaseolus vulgaris) root nodules were evaluated in an effort to identify an efficient nitrogen-fixing rhizobia strain able to improve bean germination and growth. RESULTS Common bean plants were collected from seven sites in southern Brazil, and 210 native rhizobia isolates were obtained. Evaluation of PGP traits showed that most of the rhizobia isolates were non-siderophore producers and weak indolic compounds producers. Under laboratory conditions, rhizobia isolates E15 (Rhizobium leguminosarum) and L5 (Rhizobium radiobacter) increase germination percentage, length, and dry weight of common bean and arugula (Eruca sativa) seedlings. Under greenhouse conditions, common bean plants inoculated with the rhizobia isolates VC28 and L15 (both Rhizobium fabae) presented the highest nodule number and shoot dry matter, while VC28 also presented the highest values of shoot nitrogen and potassium. Isolate L17 presented highly effective N fixation, even with reduced nodulation. CONCLUSION These new rhizobia isolates are attractive PGP alternatives to chemical fertilizers. © 2015 Society of Chemical Industry.