COMPARISON OF THE POTENCY OF 3 BRAZILIAN BOTHROPS ANTIVENOMS USING INVIVO RODENT AND INVITRO ASSAYS
COMPARISON OF THE POTENCY OF 3 BRAZILIAN BOTHROPS ANTIVENOMS USING INVIVO RODENT AND INVITRO ASSAYS
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DOI：
10.1016/0041-0101(92)90438-b
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发表时间：
1992-10-01
期刊：
影响因子：
2.8
通讯作者：
WARRELL, DA
中科院分区：
文献类型：
--
作者：
LAING, GD;THEAKSTON, RDG;WARRELL, DA
采用标准的世卫组织啮齿动物体内和体外测定法，比较了三种巴西多特异性Bothrops抗蛇毒血清中和汇集的整个Bothrops jararaca毒液的主要毒液活性的能力。在体积基础上，抗蛇毒血清在中和小鼠的致死活性方面同样有效，在中和毒液引起的出血、坏死和促凝活性方面只有微小的差异。抗蛇毒血清中和去纤维蛋白生成的效果各不相同。然而，当等量的抗蛇毒IgG进行比较时，发现FUNED抗蛇毒血清能最好地中和致死性、出血、坏死和纤维蛋白原凝血活性。Vital Brazil和FUNED抗蛇毒血清在中和血浆凝血活性方面同样有效，但Vital Brazil抗蛇毒血清在中和去纤原作用方面更有效。
Three Brazilian polyspecific Bothrops antivenoms were compared using standard W.H.O. rodent in vivo and in vitro assays of their ability to neutralize the principal venom activities of pooled whole Bothrops jararaca venom. On a volume basis, the antivenoms were equally effective in neutralizing lethal activity in mice, and there were only minor differences in their ability to neutralize venom-induced haemorrhage, necrosis and procoagulant activity. Antivenom efficacy in neutralizing defibrinogenation varied. However, when equal amounts of antivenom IgG were compared, it was found that the FUNED antivenom best neutralized lethality, haemorrhage, necrosis and fibrinogen clotting activity. Vital Brazil and FUNED antivenoms were equally effective in neutralizing plasma coagulant activity but Vital Brazil antivenom was the more effective in neutralizing defibrinogenation.