Hemocyte aggregation in the oyster Crassostrea gigas: In vitro measurement and experimental modulation by xenobiotics
Hemocyte aggregation in the oyster Crassostrea gigas: In vitro measurement and experimental modulation by xenobiotics
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DOI：
10.1016/s0300-9629(97)00017-0
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发表时间：
1997-11-01
影响因子：
2.3
通讯作者：
Oubella, R
中科院分区：
文献类型：
--
作者：
Auffret, M;Oubella, R
水生无脊椎动物的血淋巴是许多环境污染物的目标。细胞免疫应答的不同事件构成了用于开发敏感生物测定的潜在模型。在这里，描述了一种新的方法来量化在体外自发聚集的太平洋牡蛎血细胞。初步分析表明，当细胞在低温或高温下孵育时，聚集指数发生改变。在培养基中加入螯合剂EDTA或药物咖啡因是强有力的抑制剂。在污染的海洋沃茨中发现的外源性物质[三丁基锡（TBT）和微量金属]的影响，通过暴露于细胞或整个个体进行了测试，并显示为有效的潜在抑制剂。对镉的灵敏度高于对铜的灵敏度。实验室中的个人接触农药或三丁基锡化合物在峰值环境浓度导致血细胞聚集的适度变化，这种反应可以，但是，这些浓度与其他污染物或与物理化学因素在现场的协同效应增强。自发性血细胞聚集的改变被认为反映了血细胞在维持稳态甚至内部防御方面的功能受损。这种简单的测定方法可能构成一个额外的工具，实验外源性物质在软体动物中的毒性作用。(C)1997年爱思唯尔科学公司
The hemolymph of aquatic invertebrates is the target of many environmental contaminants. Different events of the cellular immune response constitute potential models for the development of sensitive bioassays. Here, a new methodology is described to quantify in vitro spontaneous aggregation by Crassostrea gigas hemocytes. Preliminary assays indicated that the aggregation index was altered when the cells were incubated at low or high temperature. The chelator EDTA or the drug caffeine added to the medium were powerful inhibitors. The effects of xenobiotics actually found in polluted marine waters [tributyltin (TBT) and trace metals] were tested by exposure of either the cells or whole individuals and appeared as efficient potential inhibitors. A higher sensitivity to cadmium than to copper has been revealed. Exposure of individuals in the laboratory to pesticides or TBT at peak environmental concentrations resulted in moderate changes in hemocyte aggregation, a response that could, however, be enhanced by synergistic effects of these concentrations with other contaminants or with physicochemical factors in the field. The alteration of spontaneous hemocyte aggregation is thought to reflect an impairment of hemocyte functions in homeostasis keeping and even in internal defense. This simple assay may constitute an additional tool to experiment the toxic effects of xenobiotics in molluscs. (C) 1997 Elsevier Science Inc.