SIMPLE METHOD TO DETERMINE FAT CELL SIZE AND NUMBER IN 4 MAMMALIAN SPECIES
SIMPLE METHOD TO DETERMINE FAT CELL SIZE AND NUMBER IN 4 MAMMALIAN SPECIES
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DOI：
10.1152/ajplegacy.1971.221.3.850
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发表时间：
1971-01-01
影响因子：
--
通讯作者：
FERTIG, JW
中科院分区：
文献类型：
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作者：
DIGIROLAMO, M;MENDLINGER, S;FERTIG, JW
迪吉罗拉莫，马里奥，谢尔顿·门德林格和约翰·w·费蒂格。一种测定四种哺乳动物细胞大小和数量的简单方法。点。[j] .中国生物医学工程学报，2016,31(3):357 - 357。1971.-通过直接显微镜测定分离脂肪细胞直径的方法，从四种哺乳动物的不同脂肪库中提取的脂肪组织在平均脂肪细胞大小（直径和体积）、脂肪细胞大小的相对分散度和脂肪细胞数量方面进行了表征。对所涉及的各种操作的精度以及对脂肪细胞大小和数量的最终估计进行了评估，发现在可接受的范围内。研究了不同发育阶段的自由喂养大鼠、仓鼠、豚鼠和狗的脂肪细胞形态。在每个物种中，给定脂肪库中脂肪细胞的增大导致脂肪细胞数量在大小上更加均匀。在大鼠的早期发育阶段（2个月大），在脂肪器官的四个不同位置发现了相当大的脂肪细胞大小和数量差异。这些发现表明，这种方法的普遍适用性，以表征脂肪组织的哺乳动物物种。此外，这项研究表明，脂肪细胞群大小的异质性是一个可测量的参数，它应该进一步作为脂肪器官扩张的指导。他研究脂肪组织；分离脂肪细胞；脂肪细胞直径；表面积和体积；脂肪细胞大小的相对分散；脂质密度脂肪器官能够在不同的年龄、内分泌状态和能量平衡条件下显著地扩张和收缩（1,8,13,21）。脂肪组织的脂质/蛋白质比值随着脂肪的增加而增加，随着脂肪量的减少而降低（1,8）。脂肪组织的这种独特能力使得比较来自同一动物、同一物种或不同物种的动物的脂肪组织片段的代谢活性变得困难。参考单位，如脂肪组织的湿重、脂质、蛋白质和DNA含量，在提供足够的比较基础方面存在问题（19）。因此，近年来发展了各种方法来测定脂肪组织中脂肪细胞的大小和数量（2,3,6,11,12,14,15,25 -27），以便相等数量的脂肪细胞可以作为比较两个或多个脂肪组织片段代谢活性的基础。此外，这些方法允许研究脂肪细胞的大小和数量在生理和病理条件下对脂肪器官扩大的贡献（4,12,16）。
DI GIROLAMO, MARIO, SHELDON MENDLINGER, AND JOHN W. FERTIG. A simple method to determine fa t cell size and number in four mam-malian species. Am. J. Physiol. 221 (3): 850458. 1971.-Adipose tissue from various fat depots in four mammalian species was characterized in terms of mean fat cell size (diameter and volume), relative dispersion in fat cell size, and fat cell number by a method based on direct microscopic determination of the diameter of isolated fat cells. The degree of precision of the various operations involved and of the final estimates of fat cell size and number were evaluated and found to be within acceptable limits. The morphology of the adipose cells was studied in ad libitum-fed rats, hamsters, guinea pigs, and dogs at different stages of development. In each species, enlargement of the fat cells in a given adipose depot led to fat cell populations that were more homoqenous in size. In the rat, at an earlier stage of development(2 mdnths of age), considerable interdepot differences were found with regard to fat cell size and number in four different locations of the adipose organ. These findings indicate the general applicability of this methodology to the characterization of adipose tissue in mammalian species. In addition, this study suggests that heterogeneity in size of a fat cell population is a measurable parameter which should further as a guide to the expansion of the adipose organ. be studied adipose tissue; isolated fat cells; fat cell diameter; surface area and volume; relative dispersion in fat cell size; lipid densityTHE ADIPOSE ORGAN is capable of expanding and contracting markedly in mass under different conditions of age, endocrine status, and energy balance (1, 8, 13, 2 1). The lipid/protein ratio of adipose tissue increases with adiposity and decreases with reduction in the adipose mass (1, 8). This unique capacity of adipose tissue has made difficult the comparison of the metabolic activity of fragments of adipose tissue from the same animal, from animals of the same species, or from different species. Units of reference such as wet weight, lipid, protein, and DNA content of adipose tissue have been found to be questionable in providing an adequate basis for comparison(19). For this reason, various methods have been developed in recent years for the determination of adipose cell size and number in adipose tissue (2, 3, 6, 11, 12, 14, 15, 25-27) so that an equal number of fat cells could form the basis for comparison of the metabolic activity of two or more fragments of adipose tissue. In addition, these methods have permitted the study of the contribution of the fat cell size and number to the enlargement of the adipose organ in physiological and pathological conditions (4, 12, 16).